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| Regulation of eight avr genes by hrpG and hrpX in Xanthomonas campestris pv. campestris and their role in pathogenicity | |
| 作者姓名： | XU Rongqi LI Xianzhen WEI Hongyu JIANG Bole LI Kai HE Yongqiang FENG Jiaxun TANG Jiliang |
| 作者单位： | 1. Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization; 2. The Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering; 3. College of Life Science and Technology, Guangxi University, Nanning 530004, China |
| 基金项目： | 国家自然科学基金;教育部资助项目;广西自然科学基金 |
| 摘 要： | Eight putative avirulence genes in Xanthomonas campestris pv. campestris (Xcc) strain 8004 were characterized by Tn5gusA5 mutagenesis and gene expression analysis. The virulence test of mutants on Chinese radish showed that all mutants in individual avr genes except avrBs2 mutant were not significantly different from the wild type in virulence. The avrBs2 mutant showed reduced virulence and bacterial growth in planta. Gene expression analysis using β-glucuronidase as reporter indicated that avrBs1.1，avrBs1，avrXccB，avrXccC，avrXccE1 were regulated by hrpG, whereas avrXccA1, avrXccA2 and avrBs2 were not. RT-PCR analysis showed that all hrpG-regulated genes except avrBs1 were also regulated by hrpX. In addition, it was demonstrated that avrBs1 was responsible for elicitation of a type III dependent hypersensitive reaction (HR) on nonhost plant pepper ECW-10R, and wild type Xcc 8004 was unable to cause HR on pepper ECW-20R. |
| 关 键 词： | Xanthomonas campestris pv. campestris avirulence gene virulence expression analysis. |
Regulation of eight avr genes by hrpG and hrpX in Xanthomonas campestris pv. campestris and their role in pathogenicity |
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| XU Rongqi,LI Xianzhen,WEI Hongyu,JIANG Bole,LI Kai,HE Yongqiang,FENG Jiaxun,TANG Jiliang.Regulation of eight avr genes by hrpG and hrpX in Xanthomonas campestris pv. campestris and their role in pathogenicity[J].Progress in Natural Science,2006,16(12):1288-1294. | |
| Authors: | XU Rongqi LI Xianzhen WEI Hongyu JIANG Bole LI Kai HE Yongqiang FENG Jiaxun TANG Jiliang |
| Institution: | 1. Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization; College of Life Science and Technology, Guangxi University, Nanning 530004, China 2. College of Life Science and Technology, Guangxi University, Nanning 530004, China 3. The Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering 4. The Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering; College of Life Science and Technology, Guangxi University, Nanning 530004, China 5. Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization; The Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering |
| Abstract: | Eight putative avirulence genes in Xanthomonas campestris pv. campestris (Xcc) strain 8004 were characterized by Tn5gusA5 mutagenesis and gene expression analysis. The virulence test of mutants on Chinese radish showed that all mutants in individual avr genes except avrBs2 mutant were not significantly different from the wild type in virulence. The avrBs2 mutant showed reduced virulence and bacterial growth in planta. Gene expression analysis using β-glucuronidase as reporter indicated that avrBs1 . 1, avrBs1,avrXccB, avrXccC, avrXccE1 were regulated by hrpG, whereas avrXccA1, avrXccA2 and avrBs2 were not. RT-PCR analysis showed that all hrpG-regulated genes except avrBs1 were also regulated by hrpX. In addition, it was demonstrated that avrBs1 was responsible for elicitation of a type Ⅲ dependent hypersensitive reaction (HR) on nonhost plant pepper ECW-10R, and wild type Xcc 8004 was unable to cause HR on pepper ECW-20R. |
| Keywords: | Xanthomonas campestris pv campestris avirulence gene virulence expression analysis |
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