| 荧光标记的叶酸修饰壳聚糖纳米载体研制 |
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| 引用本文： | 金鑫,张阳德,王吉伟,张丽华,杨璞,张旭,胡玉,于丽. 荧光标记的叶酸修饰壳聚糖纳米载体研制[J]. 中南大学学报(自然科学版), 2010, 41(1) |
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| 作者姓名： | 金鑫 张阳德 王吉伟 张丽华 杨璞 张旭 胡玉 于丽 |
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| 作者单位： | 中南大学,卫生部肝胆肠外科研究中心,湖南,长沙,410008 |
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| 基金项目： | 国家十五科技攻关计划项目 |
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| 摘 要： | 制备荧光标记的叶酸偶联壳聚糖纳米粒,为抗肿瘤药物给药系统提供载体材料。通过叶酸活性酯与壳聚糖上的氨基反应,使叶酸与壳聚糖偶联。将异硫氰基荧光素与叶酸偶联壳聚糖进行化学嫁接,以离子交联法制成具有荧光的叶酸偶联壳聚糖纳米粒,并与肝癌HepG2细胞进行体外细胞实验。实验结果表明:叶酸活性酯用量和反应温度及试剂滴加速度是影响偶联比的主要因素;在叶酸活性酯与壳聚糖用量质量比为1:1,反应温度为30℃,滴加速度为2mL/min,反应时间为12h的条件下可得到偶联稳定的叶酸偶联壳聚糖;所制得的纳米粒粒径为290nm,形态规则,细胞荧光效果明显;此方法能用于制备荧光标记的叶酸修饰壳聚糖纳米粒载体。
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| 关 键 词： | 叶酸 壳聚糖 纳米粒 异硫氰基荧光素 |
Preparation and characterization of fluorimetric folate-coupled chitosan nano-carrier |
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| JIN Xin,ZHANG Yang-de,WANG Ji-wei,ZHANG Li-hua,YANG Pu,ZHANG Xu,HU Yu,YU Li. Preparation and characterization of fluorimetric folate-coupled chitosan nano-carrier[J]. Journal of Central South University:Science and Technology, 2010, 41(1) |
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| Authors: | JIN Xin ZHANG Yang-de WANG Ji-wei ZHANG Li-hua YANG Pu ZHANG Xu HU Yu YU Li |
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| Affiliation: | JIN Xin,ZHANG Yang-de,WANG Ji-wei,ZHANG Li-hua,YANG Pu,ZHANG Xu,HU Yu,YU Li (National Hepatobiliary , Enteric Surgery Research Center,Central South University,Changsha 410008,China) |
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| Abstract: | Folate-coupled chitosan was prepared as a delivery system material for anticancer drug by the reaction of the activated folate ester with the amine group on the chitosan. The compound of folate-coupled chitosan grafted on fluorescein isothiocyanate (FITC) by chemical bond was prepared. Then FITC-labelled nanoparticles were prepared by ionically cross-linking method, and reaction with HepG2 and cells in vitro experiments. The results show that the amount of the activated folate ester, the speed of adding age... |
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| Keywords: | folate chitosan nanoparticles fluorescein isothiocyanate |
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