| 黄花蒿叶片植株再生体系的建立 |
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| 引用本文： | 刘春朝,胡楠,尚富德.黄花蒿叶片植株再生体系的建立[J].河南大学学报(自然科学版),2007,37(6):626-628. |
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| 作者姓名： | 刘春朝 胡楠 尚富德 |
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| 作者单位： | 1. 河南大学,生命科学学院,农业生物技术研究所,河南,开封,475001
2. 南阳理工学院,生物与化学工程学院,河南,南阳,473000 |
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| 摘 要： | 以黄花蒿叶片为材料,设计离体培养不同阶段的培养基配方.通过对培养结果进行观察、统计分析,结果表明:MS 2,4-D(2.0mg/L)对叶片诱导愈伤组织效果最好;MS 6-BA(1.0mg/L)为丛生芽分化培养基的适宜配方;无菌苗在MS NAA(0.05mg/L)的培养基上生根效果良好.
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| 关 键 词： | 黄花蒿 叶片 再生体系 |
| 文章编号： | 1003-4978（2007）06-0626-03 |
| 修稿时间： | 2007-03-15 |
Establishment of Regeneration System from the Leaves of Artemisia annua L. |
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| LIU Chun-chao,HU Nan,SHANG Fu-de.Establishment of Regeneration System from the Leaves of Artemisia annua L.[J].Journal of Henan University(Natural Science),2007,37(6):626-628. |
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| Authors: | LIU Chun-chao HU Nan SHANG Fu-de |
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| Institution: | 1 .Institute of Agricultural Biotechnology, College of Life Science, Henan University, Henan Kaifeng 475001 ,China; 2 .College of Biology and Chemical Engineering, Nanyang Institute of Science and Technology, Henan Nanyang 473000 ,China |
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| Abstract: | The leaves of Artemisia annua L. were taken as explants,and the optimum media for each culture stage were selected. The results showed that the medium MS 2,4-D (2.0 mg/L ) was the best for inducing callus from leaf ;medium MS 6-BA (1.0 mg/L) was the most suitable for propagation ;medium MS NAA (0.05 mg/L) was the optimum for rooting. |
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| Keywords: | Artemisia annua L leaves regeneration system |
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