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Injection of a soluble sperm fraction into sea-urchin eggs triggers the cortical reaction |
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| Authors: | B. Dale L. J. DeFelice G. Ehrenstein |
| Affiliation: | (1) Laboratory of Biophysics, NINCDS, National Institutes of Health, 20205 Bethesda, Maryland, USA;(2) Department of Anatomy, Emory University, 30322 Atlanta, Georgia, USA;(3) Marine Biological Laboratory, 02543 Woods Hole, Massachusetts, USA |
| Abstract: | Summary Fertilization membranes from around unfertilized sea-urchin eggs after microinjection of a soluble spermatozoa fraction isosmotic with seawater. This demonstrates that the spermatozoon contains a chemical that triggers an increase in cytosolic calcium, leading to exocytosis of cortical granules. It also demonstrates that the triggering mechanism does not require an externally-activated egg-membrane process. Further experiments show that the chemical trigger is not calcium. |
| Keywords: | Sea-urchin eggs Lytechnius pictus fertilization membrane spermatozoa fraction triggering mechanism |
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