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丽格海棠的组织培养与快速繁殖研究
引用本文:远凌威,袁正仿,张苏锋.丽格海棠的组织培养与快速繁殖研究[J].信阳师范学院学报(自然科学版),2002,15(2):219-221.
作者姓名:远凌威  袁正仿  张苏锋
作者单位:信阳师范学院,生物系,河南,信阳,464000
基金项目:河南省科技攻关基金资助项目 (0 0 10 5 0 0 31)
摘    要:以丽格海棠的茎状、叶柄、叶片为外植体进行组织培养。最佳培养基:(1)诱导培养基MS+6-BA4.0mg/L(单位下同)+IBA1.0;(2)增殖培养基MS+6-BA0.5+IAA0.2;(3)生根培养基MS+NAA0.2.生根壮苗培养50d左右,经移栽,成活率达92%左右。

关 键 词:丽格海棠  组织培养  快速繁殖  愈伤组织  增殖培养基  生根培养基  诱导培养基  外植体  增殖培养基
文章编号:1003-0972(2002)02-0219-03

Study on tissue culture and rapid propagation of Begonia elalior
YUAN Ling wei,YUAN Zheng fang,ZHANG Su feng.Study on tissue culture and rapid propagation of Begonia elalior[J].Journal of Xinyang Teachers College(Natural Science Edition),2002,15(2):219-221.
Authors:YUAN Ling wei  YUAN Zheng fang  ZHANG Su feng
Abstract:Tap buds,leaves and leaf stalks of Begonia elalior,after sterilized,were used as tissue culture explants.The most appropriate culture medium in culture stages were(1)MS+6 BA4.0 mg/L+IBA1.0 mg/L for germination;(2)MS+6 BA 0.5 mg/L+IAA 0.2 mg/L for rapid propagation;(3)MS+NAA 0.2 mg/L for rooting.Plantlets were transplanted after rooting culture about 50 days.The survival rate of transplanted was to 92%.
Keywords:Begonia elalior  tissue culture  rapid propagation
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