| 龙葵碱对HepG2细胞NAT1酶活性的影响 |
| |
| 引用本文： | 高世勇,苏怡君,季宇彬.龙葵碱对HepG2细胞NAT1酶活性的影响[J].哈尔滨商业大学学报(自然科学版),2010,26(5). |
| |
| 作者姓名： | 高世勇 苏怡君 季宇彬 |
| |
| 作者单位： | 哈尔滨商业大学生命科学与环境科学研究中心药物研究所博士后科研工作站;黑龙江中医药大学中药学博士后科研流动站;国家教育部抗肿瘤天然药物工程研究中心; |
| |
| 基金项目： | 黑龙江省博士后基金资助项目，教育部博士点基金项目 |
| |
| 摘 要： | 探讨龙葵碱对HepG2人肝癌细胞的N-乙酰基转移酶1(NAT1)酶活性的影响.采用高效液相色谱法(HPLC),以对氨基苯甲酸(PABA)为底物,以PABA被NAT1乙酰化为乙酰对氨基苯甲酸(Ac-PABA)的量反应NAT1酶的活性.观察不同质量浓度、不同时间龙葵碱对完整HepG2细胞NAT1酶活性的影响;龙葵碱对HepG2细胞细胞质中NAT1酶活性的影响.结果表明,在NAT1酶活性测定中,龙葵碱能显著降低HepG2完整细胞NAT1的活性;龙葵碱能够降低HepG2细胞质内NAT1的活性,且作用具有剂量依赖性;随着时间的增加NAT1转化产物的量逐渐增加,但龙葵碱能显著降低同一时段NAT1的活性.提示龙葵碱通过抑制HepG2细胞中NAT1的活性是龙葵碱抑制人肝癌细胞HepG2增殖的作用机制之一.
|
| 关 键 词： | 龙葵碱 HepG2人肝癌细胞 NAT1酶 |
Effect of solanine on N-acetylaransferase activity in HepG2 cell |
| |
| GAO Shi-yong,SU Yi-jun,JI Yu-bin.Effect of solanine on N-acetylaransferase activity in HepG2 cell[J].Journal of Harbin University of Commerce :Natural Sciences Edition,2010,26(5). |
| |
| Authors: | GAO Shi-yong SU Yi-jun JI Yu-bin |
| |
| Institution: | GAO Shi-yong1,2,3,SU Yi-jun1,JI Yu-bin1,3(1.Post-doctoral Research Station of Institute of Materia Medica of Center of Research and Development on Life Sciences and Environment Sciences of Harbin University of Commerce,Harbin 150076,China,2.Post-doctoral Research Stations in Chinese Medicine,Heilongjiang University of Chinese Medicine,Harbin 150040,3.MOE Engineering Research Center of Natural Anticancer Drugs,Harbin University of Commerce,China) |
| |
| Abstract: | To explore the effect of solanine on N-acetyltransferase1(NAT1) activity in HepG2 cell.Employing HPLC,using PABA as substrate,taking concentration of PABA as concentration of substrate,in intact HepG2 cells and their cytoplasm,taking the speed of PABA being acetylated to Ac-PABA by NAT1 as the activity of NAT1.The results show that solanine could inhibit the activity of NAT1 in intact HepG2 cell and the cytoplasm.So solanine acts to cytotoxicity to HepG2 cell by inhibiting the NAT1 activity. |
| |
| Keywords: | solanine HepG2 cell N-acetyltransferase 1(NAT1) |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
