补阳还五汤与高血压病气虚血瘀证“方证相关”的蛋白质组学研究

揭示补阳还五汤与高血压病气虚血瘀证"方证相关"的现代生物学基础,制作高血压病气虚血瘀证细胞模型。用补阳还五汤含药血清干预细胞模型,利用双向凝胶电泳(2-DE)及基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS/MS)技术筛选及鉴定差异表达的蛋白点。并对其进行生物学分析。结果高血压病气虚血瘀证组与健康对照组比较,差异蛋白质点有30个。其中,有16个蛋白上调,14个蛋白质下调。补阳还五汤组与高血压病气虚血瘀证组比较,差异蛋白质点有14个。其中,有9个蛋白上调,5个蛋白质下调。MALDI-TOF-MS/MS鉴定出:高血压病气虚血瘀证组与健康对照组差异蛋白点共有8个蛋白被成功鉴定出来。表达上调的蛋白有肽基脯氨酰异构酶A、肽基脯氨酸顺反异构酶A样1亚型、真核翻译起始因子5A-1 B亚型、微管蛋白beta-2C、CRA_b亚型、3-羟酰辅酶A脱氢酶2型异构体2。表达下调的蛋白有丙酮酸激酶同工酶M1亚型、抑制蛋白-1、抑制蛋白-1 1亚型、补阳还五汤组与高血压病气虚血瘀证组差异蛋白点共有3个蛋白被成功鉴定出来。表达上调的蛋白有丙酮酸激酶CRA_c亚型、热休克蛋白27;表达下调的蛋白有膜联蛋白A1,CRA_b亚型。这些蛋白多与促进或抑制细胞凋亡有关。说明高血压病气虚血瘀证存在着细胞凋亡,补阳还五汤可以纠正高血压病气虚血瘀证引起的细胞凋亡。这些差异蛋白可以作为高血压病气虚血瘀证的标志蛋白或补阳还五汤的作用靶点,抑制细胞凋亡可能是补阳还五汤与高血压病气虚血瘀证"方证相关"的现代生物学基础之一。

Bu Yang Huan Wu Decoction and Hypertension’s Qi Deficiency Blood Stasis Syndrome Correlation Research by Proteomics

To reveal modern biological basis of Bu Yang Huan Wu Decoction(BYHWD) and hypertension’s Qi deficiency blood stasis(QDBS) syndrome correlation;the production of hypertension qi deficiency blood stasis cell model,Bu Yang Huan Wu Decoction containing serum intervene cell model,using two-dimensional gel electrophoresis(2-DE) and matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF-MS/MS) screening and identification of differentially expressed protein spots,and its biological analysis.It is resulted that hypertension QDBS group compare to the healthy control group,has 30 differential protein spots.Among these,16 upregulation and 14 protein down;BYHWD group and the hypertension BYHWD group comparison,there are 14 differential protein spots.Of these,9 upregulation,5 proteins down.MALDI-TOF-MS/MS identified: hypertension QDBS syndrome group and healthy control group showed protein spots were a total of eight proteins have been successfully identified.Upregulation of protein peptide prolyl isomerase A,peptidyl proline cis-trans isomerase A-like subtype 1,eukaryotic translation initiation factor 5A-1 B subtype,tubulin beta-2C,CRA_b subtypes,3-hydroxy-coenzyme A dehydrogenase-2 isomer 2;expression lowered the protein pyruvate kinase isoenzyme M1 subtype,inhibition of protein-1,inhibition of protein-1 1 subtype.BYHWD group and the hypertension QDBS group differences in protein spots from three proteins were successfully identified.Upregulated protein the pyruvate the kinase CRA_c subtypes,heat shock protein 27,downregulation of the protein annexin A1 CRA_b subtypes.These proteins promote or inhibit apoptosis.It is conclused that the hypertension QDBS exist apoptosis,BYHWD can correct hypertension QDBS induced apoptosis,these different proteins can act as hypertension QDBS’ s marker proteins or BYHWD’s effect target,inhibition of apoptosis may be one of the biological basis of BYHWD and hypertension QDBS correlation.