| 交流阻抗技术研究DNA与天青Ⅰ的相互作用 |
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| 引用本文： | 黄小清,柴雅琴,袁若,周路,王秀玲,李群芳.交流阻抗技术研究DNA与天青Ⅰ的相互作用[J].西南师范大学学报(自然科学版),2003,28(6):933-936. |
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| 作者姓名： | 黄小清 柴雅琴 袁若 周路 王秀玲 李群芳 |
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| 作者单位： | 西南师范大学,化学化工学院分析科学研究所,重庆,400715 |
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| 基金项目： | 国家自然科学基金资助项目(29705001)，教育部优秀青年教师资助计划，重庆市自然科学基金资助项目. |
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| 摘 要： | 将天青Ⅰ自组装在金电极表面，以交流阻抗技术和光谱技术研究了天青Ⅰ自组装膜与DNA分子的相互作用．天青Ⅰ与DNA作用使电极的本体阻抗发生变化并结合光谱分析技术研究，发现这种作用既包括静电作用又包括镶嵌作用．该方法不仅可以反映DNA与小分子间发生了作用，同时还可以对DNA进行半定量分析，是一种快速、简便、廉价的检测DNA与小分子相互作用的新方法．
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| 关 键 词： | 交流阻抗 DNA 天青Ⅰ 自组装膜 电极 核酸 生物染色剂 |
| 文章编号： | 1000-5471(2003)06-0933-04 |
| 修稿时间： | 2003年5月5日 |
Studies on the Interaction Between DNA and Azure by AC Impedance Methods |
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| HUANG Xiao-qing,CHAI Ya-qin,YUANRuo,ZHOULu,WANG Xiu-ling,LI Qun-fang School of Chemistry and Chemical Engineering,Southwest China Normal University,Chongqing ,China.Studies on the Interaction Between DNA and Azure by AC Impedance Methods[J].Journal of Southwest China Normal University(Natural Science),2003,28(6):933-936. |
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| Authors: | HUANG Xiao-qing CHAI Ya-qin YUANRuo ZHOULu WANG Xiu-ling LI Qun-fang School of Chemistry and Chemical Engineering Southwest China Normal University Chongqing China |
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| Institution: | HUANG Xiao-qing,CHAI Ya-qin,YUANRuo,ZHOULu,WANG Xiu-ling,LI Qun-fang School of Chemistry and Chemical Engineering,Southwest China Normal University,Chongqing 400715,China |
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| Abstract: | The electrochemical characteristics of DNA were studied on an electrode modified with azure. AC impedance method and UV-vis spectra were used to measure the interaction between DNA and azure. From the experiment results, we can see that the semicircle part of the AC impedance was enlarged dramatically after the azure modified electrode had an interaction with DNA. It is sure that there was interaction between DNA and azure. This interaction consists of static action and besetment action, which was also certified by UV-Vis spectra. There is also linear between the concentration of DNA and the impedance, which can be used as a half quantified analysis to DNA. AC impedance is proved to be a new, rapid and simple way to detect the interaction of DNA and small molecule. |
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| Keywords: | AC impedance azure self-assembled films |
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