含ACC脱氨酶PGPR菌株的分离及其对燕麦耐盐性的影响

本文采用富集筛选法,以1-氨基环丙烷-1-羧酸(1-aminocyclopropane-1-carboxylate,ACC)为唯一氮源,从白城农科院实验地燕麦根际土壤中分离出2株具有ACC脱氨酶活性的细菌。其中菌株7ACC9-1的ACC脱氨酶酶活性较高,并用此菌株处理燕麦种子,在不同浓度的碳酸氢钠溶液的碱胁迫下进行燕麦种子的萌发实验和初生苗的生长试验,测定燕麦种子的发芽率、燕麦初生苗的根长和苗高。结果表明:燕麦种子用菌株处理后,发芽率显着提高;同时,在各浓度的碱胁迫下,燕麦处理组的初生苗根长和苗高均显着地优于对照组。说明具ACC脱氨酶活性的7ACC9-1能够有效地缓解燕麦种子在盐碱胁迫下萌发及其在初生苗生长阶段所受到的危害。

The Isolation of PGPR Strains with ACC Deaminase and Their Influences on the Resistance of Oat

Two PGPR strains with ACC deaminase activity was isolated in the soil of oat roots in BaiCheng Academy of Agricultural Science by using enriching and screening method which took the 1-aminocyclopropane-1-carboxylate as the only source of the nitrogen.The 7ACC9-2’s ACC deaminase activity is higher than another,and it was used to treat the oat seeds,which were used in the experiment of the oat seeds germination and growth under different concentration sodium bicarbonate,measuring seed germination rate,seedling height and root length.The results showed that the germination rate was significantly increased by 7ACC9-2,and seedling height and root length are also significantly better than check group under different concentration sodium bicarbonate.The 7ACC9-2 can effectively solve the problem of the salinization of oat seed germination and harms of growth stage.