Effect of matrix metallo-proteinase-26 (MMP-26) during embryo implantation in the mouse |
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Authors: | Jian?Zhang Sumin?Li Yongqiang?Tian Yunge?Zhao Amy?Sang?Qing-Xiang Email author" target="_blank">Enkui?DuanEmail author |
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Institution: | (1) State Key Lab of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100080 Beijing, China;(2) Department of Chemistry and Biochemistry and Institute of Molecular Biophysics, Florida State University, 32306 Tallahassee, Florida, USA |
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Abstract: | Matrix metalloproteinase-26 (MMP-26, endometase and matrilysin-2), a novel member of the MMPs family, is detected not only
in the placenta and uterus, but is widely expressed in malignant tumors from different sources as well as in diverse tumor
cell lines. However, the function of MMP-26 in the reproductive system has never been reported. Expression of MMP-26 in mouse
embryos and the function of the MMP-26 antibody during mouse embryo implantation was examined for the first time by injecting
the uterine horn, immunohistochemistry,in situ hybridization, co-culture of mouse blastocysts and uterine monolayer epithelial cells, Western blot, RT-PCR, Northern blot
and zymography. Our results show that there is strong expression of MMP-26 mRNA and protein in the mouse embryo. Furthermore,
the MMP-26 antibody dramatically inhibited mouse embryo implantation and significantly inhibited adhesion and outgrowth of
mouse blastocysts onin vitro uterine monolayer epithelial cells. At the same time, the MMP-26 antibody inhibited the expression of integrin αV mRNA and
protein in a dose-dependent manner. These data suggest that MMP-26 may play a role in some of the tissue-remodeling events
associated with the invasion of the endometrium by trophoblast cells and facilitate successfully embryo implantation. |
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Keywords: | matrix metalloproteinases (MMPs) MMP-26 embryo implantation mouse |
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