| 甜荞愈伤组织分化研究 |
| |
| 引用本文： | 郝建平.甜荞愈伤组织分化研究[J].山西大学学报(自然科学版),2007,30(3):400-405. |
| |
| 作者姓名： | 郝建平 |
| |
| 作者单位： | 山西大学,生命科学与技术学院,山西,太原,030006 |
| |
| 摘 要： | 研究了龙山荞、浦江荞、美国荞和牡丹荞四种甜荞子叶和下胚轴的愈伤组织形成与分化的影响因子.结果表明：17.8μmol/L～26.6μmol/L（4.0 mg/L～6.0 mg/L）的BA与0.9μmol/L（0.2 mg/L）的2,4-D配合使用可有效促进甜荞愈伤组织中芽的分化和芽的生长;高浓度的蔗糖（4.5%～6.0%）促进甜荞愈伤组织根的分化和愈伤组织的生长;在培养基中附加活性炭抑制甜荞愈伤组织分化根.
|
| 关 键 词： | 荞麦 愈伤组织分化 活性炭 蔗糖 |
| 文章编号： | 0253-2395（2007）03-0400-06 |
| 修稿时间： | 2006-04-29 |
Study on Callus Differentiation of Common Buckwheat* |
| |
| HAO Jian-ping.Study on Callus Differentiation of Common Buckwheat*[J].Journal of Shanxi University (Natural Science Edition),2007,30(3):400-405. |
| |
| Authors: | HAO Jian-ping |
| |
| Institution: | School of Life Science and Technology ,S hanxi University , Taiyuan 030006,China |
| |
| Abstract: | Calli from cotyledon and hypocotyl explants of four cultivars of common buckwheat were induced on Murashige and Skoog (MS) basal medium and the effects of plant growth regulators and sucrose concentrations on callus differentiation were investigated.The results showed that the combination of 17.8 μmol/L～26.6 μmol/L(4.0 mg/L～6.0 mg/L) of 6-benzyladenine (BA) with 0.9 μmol/L(0.2 mg/L) of 2,4-dichlorophenoxyacetic acid (2,4-D) was effective in inducing differentiation and generation of adventitious buds.Comparative studies using different sucrose concentrations (1.5 %～6.0 %) demonstrated that higher sucrose concentrations (4.5 %～6.0 %) promoted root differentiation and enhanced callus growth.Addition of activated charcoal into the culture media inhibited root differentiation. |
| |
| Keywords: | 2 4-D BA buckwheat callus differentiation 2 4-dichlorophenoxyacetic acid 6-benzyaldenine activated charcoal sucrose |
| 本文献已被 维普 万方数据 等数据库收录！ |
