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| 抗氟甲砜霉素多克隆抗体的制备与鉴定 | |
| 引用本文： | 褚文雷,崔杰,马莺,李滨胜.抗氟甲砜霉素多克隆抗体的制备与鉴定[J].黑龙江大学自然科学学报,2010,27(2). |
| 作者姓名： | 褚文雷 崔杰 马莺 李滨胜 |
| 作者单位： | 1. 哈尔滨工业大学生命科学与工程系,哈尔滨,150001 2. 哈尔滨工业大学食品科学与工程学院,哈尔滨,150090 3. 黑龙江省森林植物园引种驯化研究中心,哈尔滨15004|D |
| 基金项目： | 哈尔滨市科技攻关项目 |
| 摘 要： | 为建立氟甲砜霉素的免疫分析方法,采用混合酸酐法合成氟甲砜霉素人工抗原(FF-HS-BSA)和包被抗原(FF-HS-OVA),并利用紫外吸收光谱及红外光谱扫描方法检测到人工合成抗原偶联成功。利用合成的人工抗原免疫5只6～8周龄BALB/c小鼠,免疫8次后得到氟甲砜霉素抗血清。利用间接非竞争酶联免疫吸附测定法检测抗体效价为1∶6400,可用于进一步实验研究。 |
| 关 键 词： | 氟甲砜霉素 人工抗原 多克隆抗体 |
Preparation and identification of polyclonal antibody against florfenicol |
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| CHU Wen-lei,CUI Jie,MA Ying,LI Bin-sheng.Preparation and identification of polyclonal antibody against florfenicol[J].Journal of Natural Science of Heilongjiang University,2010,27(2). | |
| Authors: | CHU Wen-lei CUI Jie MA Ying LI Bin-sheng |
| Abstract: | In order to establish the immunoassay method of florfenicol (FF), FF was coupled with carrier protein Bovine Serum Albumin(BSA) and Ovalbumin(OVA) to prepare complete antigen FF-HS-BSA and FF-HS-OVA by mixed anhydride (MA) reaction. UV and IR methods were used to identify synthetic antigen which results showed that the reaction was suecessed. Five BALB/C mice, 6 ～ 8 weaks old, were immunized with FF-HS-BSA, and the polyclonal antibody(pAb) after 8 times immunized was got. The titer of pAb was detected by indirect ELISA, the ti-ter of FF antiserum was 1 : 6 400,it could be used in the next study. |
| Keywords: | ELISA florfenicol artificial antigen polyclonal antibody ELISA |
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