红芪水提物抗炎作用的动物实验研究

探讨红芪水提取物（Radix hedysari water extract, RHWE）对小白鼠的体外抗炎活性。选取体重20~28 g SPF级Balb/c小白鼠100只，通过小白鼠腹股沟棉球肉芽肿增生抑制试验和二甲苯致小白鼠急性耳肿胀试验检测红芪提取物抗炎效果，将小白鼠急性耳肿胀试验打下的耳片制作石蜡切片，观察其形态学变化。红芪提取物高、中、低浓度组对小白鼠腹股沟棉球肉芽肿增生抑制率分别是18.63%、15.69%和6.87%；红芪提取物高、中、低浓度组对小白鼠急性耳肿胀抑制率分别是27.90%、5.58%和2.05%；病理切片观察显示，红芪提取物低浓度组和阴性对照组弹性软骨和皮肤层之间空隙明显，真皮层内组织结构散布存在，中高浓度组耳组织相对完整，未见明显炎症反应。研究表明，红芪提取物具有一定的抗炎活性，且其抗炎活性在一定范围内与浓度呈正相关。

ANIMAL EXPERIMENTAL STUDY ON THE ANTI-INFLAMMATORY EFFECTS OF RADIX HEDYSARI WATER EXTRACT

The anti-inflammatory activity of radix hedysari water extract (RHWE) in vitro was explored. 100 mice (22-28 g SPF BALB/c) were selected to test the anti-inflammatory effect of RHWE by the auricle swelling test and cotton-pellet granuloma test, a paraffin sectioning of the ear piece was made for observing the morphological changes of ear tissue. The inhibitory rates of granuloma hyperplasia were 18.63%, 15.69% and 6.87% in high, medium and low dose groups of RHWE. The inhibition rates of auricle swelling in high, medium and low dose groups of RHWE were 27.90%, 5.58% and 2.05%. Pathological observations showed that the gap between the elastic cartilage and the skin layer was obvious in low dose group and the blank control group of RHWE there were scattered tissue structures in the dermis, the ear tissue was relatively intact in high and medium dose groups, no obvious inflammatory reaction was observed. The results showed that RHWE had some anti-inflammatory activity, which was positively correlated with its dose.