CCNB2在肾透明细胞癌中的表达意义与功能网络关系分析

分析细胞周期蛋白B2（CCNB2）在肾透明细胞癌中的表达及与临床预后的关系，建立CCNB2共表达网络并探讨其潜在的作用机制。采用GEPIA和UALCAN数据库分析CCNB2在肾透明细胞癌临床样本中的表达情况，获得CCNB2与肾透明细胞癌的病理分期及预后的关系，同时检测肾透明细胞癌及癌旁临床组织中目的mRNA的表达；通过cBioportal和String数据库分析CCNB2在肾透明细胞癌的功能作用，建立CCNB2共表达网络；DVIAD和KEGG数据库在线分析共表达网络功能及富集的信号通路。结果表明： CCNB2在肾透明细胞癌组织中高表达，并且与肾透明细胞癌的病理分期及预后呈现正相关性。从cBioportal数据库获得肾透明细胞癌患者数据，挖掘得到CCNB2表达关系密切的83个基因，建立CCNB2功能网络。进一步采用DVIAD和KEGG数据库在线分析发现富集的基因主要参与细胞周期、卵母细胞减数分裂和卵细胞成熟通路等信号通路，共表达基因功能显示为微管结合、微管运动活性和微管蛋白结合等。因此，CCNB2在肾透明细胞癌组织中显著高表达，并与患者预后、临床病理分期呈正相关，这些预示着CCNB2不仅能够作为肾透明细胞癌诊断、病理分期和预后的重要分子标志物，还是肾透明细胞癌潜在的治疗靶标。

ANALYSIS OF THE EXPRESSION SIGNIFICANCE AND FUNCTIONAL NETWORK RELATIONSHIP OF CYCLINB2 IN KIDNEY RENAL CLEAR CELL CARCINOMA

The expression of CyclinB2 (CCNB2) in Kidney renal clear cell carcinoma (KIRC) and its relationship with clinical prognosis were analyzed, CCNB2 co-expression network was established and its potential mechanism was explored. GEPIA and UALCAN databases were used to analyze whether CCNB2 was associated with pathological stage and prognosis. The target mRNA expression in clinical KIRC and adjacent tissues were compared by realtime-PCR. In order to establish CCNB2 functional network, cBioportal and string databases were used to analyze the function of CCNB2 in KIRC. The co-expression network and enriched signal pathways were analyzed by On-line analysis of DVIAD and KEGG databases. The results showed that CCNB2 was expressed highly in KIRC, which was positively correlated with the pathological stage and prognosis. cBioportal database was used to collect and analyze the data of KIRC patients. The results showed that 83 genes had osculate relationship with CCNB2. Furthermore, it was found that these enriched genes involved in several signal pathways, mainly including cell cycle, oocyte meiosis, progesterone-mediated oocyte maturation and so on. The function of these co-expressed genes were related to microtubule binding, microtubule motor activity, tubulin binding. Therefore, CCNB2 is highly expressed in KIRC and positively correlated with the prognosis and clinicopathologic stage of the patients. These results indicate that CCNB2 is not only an important molecular marker for the diagnosis, pathologic staging and prognosis of KIRC, but also a potential therapeutic target for KIRC.