| 大豆磷脂酰乙醇胺分子种组成分析 |
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| 引用本文： | 连桂香,夏海涛,刘玉芬.大豆磷脂酰乙醇胺分子种组成分析[J].齐齐哈尔大学学报(自然科学版),2004,20(1):15-17. |
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| 作者姓名： | 连桂香 夏海涛 刘玉芬 |
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| 作者单位： | 齐齐哈尔大学化学与化工学院,齐齐哈尔,161006 |
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| 摘 要： | 用高效液相色谱法(HPLC)在反相Prodigy ODS(3)柱上，以甲醇-氯仿-乙腈-水为流动相，梯度洗脱程序分析了大豆磷脂酰乙醇胺的分子种组成，蒸发光散射检测器用做HPLC峰的检测，峰面积归一化法定量。组分定性由气．相色谱(GC)测定的HPLC峰的脂肪酸组成确定。分析结果表明，大豆磷脂酰乙醇胺由9个分子种组成，其中C18：2C18：2,C18：1C18：2,C16：0C18：2，C16：0C18：1，C18：0C18：2分子种是主要组分。
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| 关 键 词： | 高效液相色谱法 大豆 磷脂酰乙醇胺 分子种 植物生物膜 |
| 文章编号： | 1007-984X(2004)01-0015-03 |
| 修稿时间： | 2003年10月6日 |
Analysis of soybean phosphatidylethanolamine molecular species using high performance liquid chromatography |
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| LIAN Gui-xiang XIA Hai-tao LIU Yu-fen.Analysis of soybean phosphatidylethanolamine molecular species using high performance liquid chromatography[J].Journal of Qiqihar University(Natural Science Edition),2004,20(1):15-17. |
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| Authors: | LIAN Gui-xiang XIA Hai-tao LIU Yu-fen |
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| Abstract: | Soybean phosphatidylethanolamine molecular species was analysis on a reverse phase Prodigy ODS (3) column by high performance liquid chromatography, gradient eluted program was performed with flow phase consisting of chloroform-methanol-acetonitrile-water. An evaporative light-scattering detector was used for HPLC quantification. Molecular species identities were assigned from fatty acid compositions determined by gas chromatography. 9 molecular species of soybean PE were found, C18:2C18:2, Cl8:1,C18:2, C16:0C18:1, C16:0C18:2, C18:0C18:2were the maior molecular species. |
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| Keywords: | High-Performance Liquid Chromatography Phosphatidylethanolamine Soybean Molecular species |
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