| 筋骨草中槲皮素成分的含量测定 |
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| 引用本文： | 陈建琴,;许雪琴.筋骨草中槲皮素成分的含量测定[J].莆田高等专科学校学报,2008(5):43-45. |
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| 作者姓名： | 陈建琴 ;许雪琴 |
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| 作者单位： | [1]莆田学院环境与生命科学系,福建莆田351100; [2]福州大学化学系,福建福州350002 |
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| 摘 要： | 采用高效液相色谱法对筋骨草中槲皮素的含量进行测定。色谱条件为：色谱柱，HypersilODS（5μm，250．0×4．0mm）；流动相，pH=2．08磷酸：甲醇为45：55；检测波长为370nm。标准曲线显示，槲皮素在2．24-22．40μg·mL^-1范围内呈良好的线性关系，回归方程为可=192．0x＋202．4，r=0．998。样品平均回收率为97．6％，RSD为1．67％，这表明本法是一种筋骨草中槲皮素的分析及含量测定的简便、快速、准确且重现性好的方法。
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| 关 键 词： | 筋骨草 槲皮素 含量 测定 高效液相色谱法 |
Determination of Quercetin in the Ajuga Decumbent by HPLC |
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| Institution: | CHEN Jian-qin, XU Xue-qin(1. Environment ＆ Life Sciences Department, Putian University, Putian Fujian 351100, China; 2. Department of Chemistry, Fuzhou University, Fuzhou Fujian 350002, China) |
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| Abstract: | Objective： To analysis and determine the content quercetin in Ajuga Decumbent. The Hypersil ODS （5μm, 250.0×4.0mm） column was used. The mobilephase consisted of methano -- Phosphoric acid solutions （ 55：45 ） with detection wavelength of 370nm. Results： Linearity of quercetin was in the range of 2.24--22.40μg.mL^-1. The rgerssion equation was y---192.0x＋202.4 with r=0.998. The average recovery rate was 97.6% and RSD was 1.67%. Conclusion： The method is simple, quick, sensitive, accurate, reproducible and can be used to determine the content quercetin in Ajuga Decumbent. |
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| Keywords: | Ajuga Decumbent quereetin content determination HPLC |
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