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| 李多酚氧化酶酶学特性的研究 | |
| 引用本文： | 史辉,;邓伯勋,;陈锡雄. 李多酚氧化酶酶学特性的研究[J]. 莆田高等专科学校学报, 2008, 0(2): 66-69 |
| 作者姓名： | 史辉, 邓伯勋, 陈锡雄 |
| 作者单位： | [1]宁德师范高等专科学校生物系,福建宁德352100; [2]华中农业大学园艺林学学院,湖北武汉430070 |
| 基金项目： | 宁德师范高等专科学校校级科研资助项目（2006Y011） |
| 摘 要： | 采用分光光度法，研究了李多酚氧化酶（PPO）的特性，包括不同温度、pH值、底物浓度和抑制剂对PPO活性的影响，以及PPO的热稳定性。结果表明：李PPO的最适酶促反应温度为30℃；最适pH值为5．5；Km为123mmol／L；Vm为0．522△OD／min；90℃热处理2min可钝化PPO的活性；抗坏血酸和亚硫酸钠为强抑制剂，质量分数为189mg／kg的亚硫酸钠和317mg／kg的抗坏血酸能有效抑制PPO的活性。 |
| 关 键 词： | 李 多酚氧化酶 特性 褐变 |
Study on Characteristics of Polyphenoloxidase in Plum |
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| Affiliation: | SHI Hui, DENG Bo-xun, CHEN Xi-xiong (1. Department of Biology, Ningde Teachers College, Ningde Fujian 352100, China; 2. College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan Hubei 430070, China ) |
| Abstract: | The Characteristics of polyphenoloxidase of plum were studied in this paper by spectrophotometry, including the effects of different temperature, pH, concentration of substrate and inhibitors on PPO activity, as well as stability to heat. The results showed that the optimum temperature of PPO was 30℃ and the optimum pH was 5.5. Kin value and Vm value of PPO were 123mmol/L and 0.522△OD/min respectively. The extinct condition of PPO was 2 minutes at 90℃ Ascorbic acid and sodium sulfite were strong inhibitors, with the effective concentration being 317mg/kg and 189mg/kg respectively. |
| Keywords: | plum polyphenoloxidase property browning |
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