| 一株碳酸钙矿化菌的分离与鉴定 |
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| 引用本文： | 张振远,李广悦,丁德馨,王永东,胡南.一株碳酸钙矿化菌的分离与鉴定[J].南华大学学报(自然科学版),2014,28(2):30-33. |
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| 作者姓名： | 张振远 李广悦 丁德馨 王永东 胡南 |
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| 作者单位： | 南华大学核资源工程学院;南华大学铀矿冶生物技术国防重点学科实验室 |
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| 基金项目： | 湖南省研究生科研创新基金资助项目(CX2013B388) |
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| 摘 要： | 基于微生物诱导碳酸钙沉积的岩土工程加固技术是一种环境友好的新技术.碳酸钙矿化菌是该技术应用的前提.为获得具有诱导碳酸钙沉积能力的菌株,采用选择性富集培养、平板分离方法从土壤中分离得到了一株具有尿素分解能力的菌株,细菌诱导产生的沉积物检测结果表明该菌株具有诱导碳酸钙沉积能力.通过形态学、革兰氏染色和16S rDNA序列同源性分析鉴定该菌株为巴斯德芽孢杆菌.
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| 关 键 词： | 岩土工程 碳酸钙矿化 分离 巴斯德芽孢杆菌 |
| 收稿时间： | 2014/1/2 0:00:00 |
Isolation and Identification of a Bacterial Strain Inducing Mineralization of Calcium Carbonate |
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| ZHANG Zhen-yuan,LI Guang-yue,DING De-xin,WANG Yong-dong and HU Nan.Isolation and Identification of a Bacterial Strain Inducing Mineralization of Calcium Carbonate[J].Journal of Nanhua University:Science and Technology,2014,28(2):30-33. |
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| Authors: | ZHANG Zhen-yuan LI Guang-yue DING De-xin WANG Yong-dong and HU Nan |
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| Institution: | ZHANG Zhen-yuan;LI Guang-yue;DING De-xin;WANG Yong-dong;HU Nan;School of Nuclear Resources Engineering,University of South China;Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy,University of South China; |
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| Abstract: | Biocementation through microbial calcium carbonate precipitation is an innovative and environmentally friendly rock and soil reinforcement technique in geotechnical engineering.The bacteria inducing mineralization of calcium carbonate is a prerequisite to implement the biological treatment process.In order to obtain the strain with ability to induce CaCO3 precipitation,a ureolytic strain was isolated from soil using selective enrichment culture and plate screening techniques.The precipites induced by this stain were examined,and the results showed it was capable of inducing calcium carbonate mineralization.The strain was identified as Sporosarcina pasteurii based on morphology,Gram stain and 16S rDNA sequence analysis. |
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| Keywords: | geotechnical engineering calcium carbonate mineralization isolation Sporosarcina pasteurii |
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