转乙肝病毒表面抗原基因烟草发根的获得

构建了乙肝病毒表面抗原基因（HBsAg）植物表达载体，通过冻融法将HBsAg 基因转入到发根农杆菌LBA1314中，采用叶盘法将HBsAg基因导入到烟草中，获得了转基因烟草发根.对转基因烟草发根的GUS检测结果表明：转HBsAg基因烟草发根可以染成蓝色，而非转基因烟草的根没有染色反应.这说明gus基因在转HBsAg基因烟草发根获得了表达.

Transformation of HBsAg Gene into Tobacco and Production of Transgenic Tobacco Hairy Roots

A plant expression carrier containing HBsAg(hepatitis B virus surface antigen)gene was constructed in a plasmid,the plasmid was mobilized into a rhizogenes Strain LBA 1314 by freeze-thaw method,the HBsAg gene was planted into tobacco by leaf disc via Agrobacterium tumefaciens LBA 4404 as a vehicle and the tobacco hairy roots were received.Histochemical staining of GUS activity was made in the transgenic tobacco hairy roots:The roots with HBsAg genes can be dyed into bule,while those without have no reflection of color.So the conclusion can acquire that the gene has its expression in the roots.