基于核磁共振的肾虚痰瘀型2型糖尿病患者血清代谢组学研究

 利用核磁共振技术(Nuclear Magnetic Resonance,NMR)探讨肾虚痰瘀型2型糖尿病患者的血清代谢变化及发生机制。对33例肾虚痰瘀型2型糖尿病患者和50例健康人血清进行核磁共振氢谱检测,通过分段积分后运用正交偏最小二乘判别(OPLS-DA)分析所采集的图谱。结果显示,肾虚痰瘀型2型糖尿病患者与健康人相比血清中多种氨基酸显著降低,包括异亮氨酸、亮氨酸、缬氨酸、丙氨酸、谷氨酸、组氨酸、酪氨酸、苯丙氨酸、1-甲基组氨酸,差异有统计学意义(P<0.05)。此外,乳酸、柠檬酸、肌酸及肌酸酐也较健康人显著降低,差异有统计学意义(P<0.05)。同时,肾虚痰瘀型2型糖尿病患者血清中α-葡萄糖及β-葡萄糖较健康人增加,差异有统计学意义(P<0.05)。由此得出结论,肾虚痰瘀型2型糖尿病患者代谢成分与健康人存在显著差异,肾虚痰瘀型2型糖尿病患者体内的三羧酸循环及糖酵解途径被抑制,蛋白质代谢异常,支链氨基酸代谢增强。

Serum Metabonomics Research on Shenxu-tanyu Syndrome Type 2 Diabetes Mellitus Based on NMR

To explore the metabolic variation and pathogenesis of type 2 diabetes mellitus of Shenxu-tanyu syndrome in Traditional Chinese Medicine (TCM) by using Nuclear Magnetic Resonance (NMR). Serum samples from 33 cases of Shenxu-tanyu syndrome type 2 diabetes mellitus patients and 50 cases of healthy people are detected and described with 1H-NMR based metabonomics, and than the data is analyzed by Orthogonal Partial Least Squares-Discriminant Analysis (OPLS-DA). The results show that comparing with the healthy people, the various kinds of amino acids of Shenxu-tanyu syndrome type 2 diabetes mellitus patients are markedly lower, including isoleucine, leucine, valine, alanine, glutamate, histidine, tyrosine, phenylalanine, and 1-methylhistidine, the differences are statistically significant (P<0.05). In addition, lactate, citrate, creatine and creatinine are also markedly lower (P<0.05). However, the α-glucose and β-glucose are increased comparing with the health people (P<0.05). It is concluded that the metabolites of shenxu-tanyu syndrome type 2 diabetics are significantly different from healthy people, their tricarboxylic acid cycle and glycolysis are inhibited, protein is metabolic disorder, and branched chain amino acid is metabolic boost.