异常黑胆质型痴呆证病结合模型建立及方药干预实验

 为了建立阿尔茨海默病(AD)异常黑胆质证病结合大鼠模型,并观察维药异常黑胆质成熟剂对其影响,采用经典方法建立异常黑胆质证模型,随后将凝集态β-淀粉样蛋白1-40(β-Amyloid 1-40,Aβ_1-40)定位注射到双侧海马,建立病证结合模型;应用异常黑胆质成熟剂方药干预,观察各组体征变化,通过Morris水迷宫和跳台仪进行行为学测试,比较各组学习记忆能力的改变.结果表明,异常黑胆质证组和AD异常黑胆质证病结合组表现出饮水量增加(P<0.01),饮食量增加(P<0.01),体重减轻(P<0.01)等;AD组、异常黑胆质证组、AD异常黑胆质证病结合组学习记忆力降低(P<0.05);AD异常黑胆质证病结合组+异常黑胆质成熟剂干预组异常黑胆质证生物表征和学习记忆力均有所改善(P<0.05).研究表明,异常黑胆质成熟剂对AD异常黑胆质证病结合组大鼠的生物表征及学习记忆能力均有一定改善作用.

The Rate Model of Alzheimer's Disease with Abnormal Savda Syndrome and the Effectness of the Related Medical Formulas

This paper establishes a rat model of the Alzheimer's disease with abnormal savda syndrome and evaluates the effectiveness of abnormal savda munziq on the disease. A rat model of the abnormal savda syndrome is first established by using the classical method, then β-Amyloid 1-40 is injected into hippocampus to establish the rat model of the Alzheimer's disease with abnormal savda syndrome. A model group is given the abnormal savda munziq, the biological characterizations and the behavioral changes are observed with the Morris water maze test and the step down test, and the learning and memorizing abilities of different model groups are compared. It is found that compared with the normal group, the daily food and water intakes are increased in the abnormal savda syndrome group and the group of the Alzheimer's disease with abnormal savda syndrome(P<0.01), the body weight is decreased in these two groups(P<0.01); the learning and memorizing abilities are weakened in the AD group, the abnormal savda syndrome group and the group of the Alzheimer's disease with abnormal savda syndrome(P<0.05);the biological characterizations, and the learning and memorizing abilities are improved in the group of the Alzheimer's disease with abnormal savda syndrome plus the abnormal savda munziq(P<0.05). Therefore, it is established that the abnormal savda munziq can improve the biological characterization and the learning and memorizing abilities of rats of the Alzheimer's disease with abnormal savda syndrome.