| 珠江口海岸带沉积物甲烷相关古菌群落结构及多样性研究 |
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| 引用本文： | 陈金全,王风平.珠江口海岸带沉积物甲烷相关古菌群落结构及多样性研究[J].云南大学学报(自然科学版),2013,35(2):240-245. |
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| 作者姓名： | 陈金全 王风平 |
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| 作者单位： | 1.云南大学 工程技术研究院,云南 昆明 650091;2.上海交通大学 生命科学技术学院,上海 200240 |
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| 基金项目： | 国家自然科学重点基金项目(40830213)；国家自然科学基金（51168043） |
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| 摘 要： | 用构建克隆文库的方法对珠江口沉积物中4个层位的甲烷相关古菌群落多样性特征进行研究.结果表明甲烷相关古菌群落结构主要可以分为3类,分别为Methanosaeta,Methanomicrobiales和Methanosarcinales/ANME,且其组成随沉积物深度的改变发生规律变化.Methanosaeta和Methanomicrobiales是属于乙酸和H2/CO2利用型甲烷产生菌,在克隆文库中的含量随沉积物深度的增加而减少;Methanosarcinales/ANME主要由甲烷氧化菌和甲基利用型甲烷产生菌组成,在克隆文库中的含量随沉积物深度的增加而增加.甲烷相关古菌群落结构的变化规律说明,在SMTZ区域及以上,甲烷产生以H2/CO2和乙酸型为主;在SMTZ区域以下,以甲基利用型为主;在SMTZ区域,主要是甲烷氧化过程,由古菌类群ANME-2a负责.
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| 关 键 词： | 珠江口沉积物 甲烷产生 甲烷氧化 |
The community structure and diversity of methanogen-related organisms in Pearl River Estuary sediment |
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| CHEN Jinquan,WANG Fengping.The community structure and diversity of methanogen-related organisms in Pearl River Estuary sediment[J].Journal of Yunnan University(Natural Sciences),2013,35(2):240-245. |
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| Authors: | CHEN Jinquan WANG Fengping |
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| Institution: | 1.Research institute of Engineering and Technology,Yunnan University,Kunming 650091,China;2.School of Life Sciences and Biotechnology,State Key Laboratory of Marine Engineering, Shanghai Jiaotong University,Shanghai,200240,China |
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| Abstract: | Based on clone and sequencing of methanogen-related 16S rRNA genes,the diversity and composition of methanogen-related archaeal communities in Pearl River Estuary sediment were investigated.The phylogenetic results suggested the methanogen-related archaeal communities in the sediment were mainly composed of three types of groups,Methanosaeta,Methanomicrobiales and Methanosarcinales/ANME respectively.The variation of detected archaeal phylotypes by depth indicated that Methanosaeta and Methanomicrobiales were predominant groups above SMTZ and the content decreased by depth;Methanosarcinales/ANME was dominant group within or below SMTZ and the content increased by depth.Methanosaeta and Methanomicrobiales mainly belong to acetate- and H2/CO2-utilizing methanogen, which indicated methanogenesis above SMTZ mainly depend on acetate-and H2/CO2 as substrates. Methanosarcinales/ANME was responsible for methane oxidation and methyl group-utilizing methanogenesis,which suggested that methane oxidation and methyl group-utilizing methanogenesis were main metabolic processes within and below SMTZ. The subgroup ANME-2a also was identified as methane oxidizer in Pearl River sediment. |
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| Keywords: | Pearl River Estuary sediment methane oxidation methanogenesis |
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