| HPLC同时测定三宝胶囊中原儿茶醛、丹参素钠和丹酚酸B含量 |
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| 引用本文： | 梁小洁,伍庆,邹晶.HPLC同时测定三宝胶囊中原儿茶醛、丹参素钠和丹酚酸B含量[J].贵州师范大学学报(自然科学版),2008,26(4). |
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| 作者姓名： | 梁小洁 伍庆 邹晶 |
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| 作者单位： | 贵州省山地环境信息系统与生态环境保护重点实验室,贵州,贵阳,550001 |
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| 摘 要： | 目的:建立HPLC法同时测定三宝胶囊中原儿茶醛、丹参素钠、丹酚酸B含量。方法:以三宝胶囊为研究对象,采用Hypersil BDS C18(5μm,250×4.6mm)色谱柱;流动相A:含0.05%磷酸的甲醇溶液,B:0.05%磷酸水溶液,梯度洗脱。流速1mL.min-1;检测波长270nm。结果:原儿茶醛、丹参素钠、丹酚酸B分别在0.024 8~0.248μg(r=0.999 5)、0.370~3.70μg(r=0.999 9)和0.252~2.52μg(r=0.999 8)范围内呈良好的线性关系;平均回收率依次为:99.3,98.6,98.8。结论:该法操作简便、快速、准确,为三宝胶囊质量评价提供了可靠方法。
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| 关 键 词： | HPLC 三宝胶囊 原儿茶醛 丹参素钠 丹酚酸B |
RP-HPLC determination of protocaechuic aldehyde sodium Danshensu and salvianolic acide B in Sanbao capsules |
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| LIANG Xiao-jie,WU Qing,ZOU Jing.RP-HPLC determination of protocaechuic aldehyde sodium Danshensu and salvianolic acide B in Sanbao capsules[J].Journal of Guizhou Normal University(Natural Sciences),2008,26(4). |
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| Authors: | LIANG Xiao-jie WU Qing ZOU Jing |
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| Abstract: | Objective:To develop a method to determine protocaechuic aldehyde,sodium Danshensu and salvianolic acide B in Sanbao capsules.Methodes:Using RP-HPLC method with Hypersil BDS C18(5μm,250mm×4.6mm)column,the mobile phase A was methanol(0.05%phosphoric acid),B was water(0.05% phosphoric acid)eluted in gradient mode.Flow rate was 1ml.min-1,the detetion wavelength was 270nm,and column temperature was 35℃.Results: The calibration curves were linear in the range of 0.024 8μg-0.248μg(r=0.999 5) for protocaechuic aldehyde,0.370μg ～3.70μg(r=0.999 9) for sodium Danshensu and 0.252μg～2.52μg(r=0.999 8) for salvianolic acide B.The average recovery of protocaechuic aldehyde was 99.3%,of sodium Danshensu was 98.6% and of salvianolic acide B was 98.8%.Conclusion :The method is convenient and appropriate and can be applied to the quality control of Sanbao capsules. |
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| Keywords: | HPLC |
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