| 东海原甲藻钙调蛋白的分离纯化及鉴定 |
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| 引用本文： | 郭彩华,刘静雯,卢珍华,詹玲玲.东海原甲藻钙调蛋白的分离纯化及鉴定[J].集美大学学报(自然科学版),2009,14(1). |
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| 作者姓名： | 郭彩华 刘静雯 卢珍华 詹玲玲 |
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| 作者单位： | 集美大学生物工程学院,福建,厦门,361021 |
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| 基金项目： | 福建省自然科学基金，厦门大学近海海洋环境科学国家重点实验室开放基金 |
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| 摘 要： | 以东海原甲藻(Prorocentrum donghaiense Lu)为材料,经热变性、离心处理,上清液依次经DEAE纤维素阴离子交换层析和苯基-Sepharose CL- 4B疏水层析分离纯化钙调蛋白(CaM).SDS-PAGE和Western blot检测结果显示:东海原甲藻中CaM的相对分子质量为16 ku.采用该实验方法首次有效地从海洋浮游植物东海原甲藻中分离纯化出钙调蛋白.
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| 关 键 词： | 东海原甲藻 钙调蛋白 疏水纯化 |
Purification and Identification of Calmodulin(CaM) from Prorocentrum donghaiense Lu |
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| GUO Cai-hua,LIU Jing-wen,LU Zhen-hua,ZHAN Ling-ling.Purification and Identification of Calmodulin(CaM) from Prorocentrum donghaiense Lu[J].the Editorial Board of Jimei University(Natural Science),2009,14(1). |
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| Authors: | GUO Cai-hua LIU Jing-wen LU Zhen-hua ZHAN Ling-ling |
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| Institution: | School of Biotechnology Engineering;Jimei University;Xiamen 361021;China |
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| Abstract: | Calmodulin(CaM) was extracted and purified from marine phytoplankton Prorocentrum donghaiense Lu cells.The supernatant dealt with the thermal denaturation and the freeze-centrifugation was purified by the diethylaminoethyl(DEAE) cellulose ion exchange chromatography and the phenyl-sepharose CL-4B hydrophobic chromatography sequentially.CaM was detected by using SDS-PAGE and western blot.The results showed that the relative molecular mass of CaM in P.donghaiense was 16 ku and the procedures used in this expe... |
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