| GRP1.8融合4CL1基因调控杨树木质素生物合成 |
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| 引用本文： | 赵艳玲,陆海,蒋湘宁.GRP1.8融合4CL1基因调控杨树木质素生物合成[J].成都大学学报(自然科学版),2012,31(2):99-102,107. |
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| 作者姓名： | 赵艳玲 陆海 蒋湘宁 |
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| 作者单位： | 1. 华侨大学化工学院,福建厦门,361021
2. 北京林业大学生物科学与技术学院,北京,100083 |
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| 基金项目： | 福建省科技厅重点科技计划 |
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| 摘 要： | 将形成层定位启动子GRP1.8与毛白杨的4-香豆酸:辅酶A连接酶基因融合,叶盘法转化毛白杨741,southern杂交检测表明融合基因已经整合到转基因杨树的基因组中.通过实时定量PCR技术测定mRNA的表达量,GC-MS分析4CL底物香豆酸的含量和定量硫酸木质素含量,分析了4CL基因的过量表达对转基因杨树生理生长的影响.实验结果表明,整合了正义4CL1的转基因植株在茎中的酶活性比野生植株增加了30%,木质素含量增加了40%.
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| 关 键 词： | -香豆酸 辅酶A连接酶 GRP.启动子 杨树 木质素生物合成 |
Regulation of Lignin Biosynthesis in Populus Tomentosa with GRP1.8 Promoter and 4CL1 Gene Constructs |
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| Institution: | ZHAO Yanling, LU Hai, (1. School ef Chenfieal Engineering, 2. School of Life Sciences and Bioteehnology＇, Huaqiao University, Xiamen 361021, China; Beijing Forestry Univemity, Beijing 100083, China) |
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| Abstract: | Plant expression binary vector was constructed by inserting vasc~ar-specific-expression promoter of the glycine-rich-protein （GRP1.8） from acacia fused 4-coumarate： CoA ligase （4CL1） gene from popu- lus tomentosa. Populus tomentosa 741 were transformed through leaf discs mediated with agrobacterium in- fection and regenerated through tissue culture. Regenerated transformed plants were molecular verified by Southern blotting. Content of mRNA was analyzed by Real-time PCR,4-coumarate acid content by GC-MS and qualitative analysis of lignin by chemistry analysis procedure. Experimental results show that compared to the stem of wild type plants,the enzyme activity is increased by 30% and the lignin content is increased by 40% in the GRP1.8-4CL1 fusion transgenic poplar. |
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| Keywords: | 4-coumamte coenzyme coenzyme A ligase GRP1 8 promoter poplar lignin biosynthesis |
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