| 血液痕迹中DNA抽提方法的比较与优化 |
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| 引用本文： | 齐麟,郜杨,向志明,张彤.血液痕迹中DNA抽提方法的比较与优化[J].河南大学学报(自然科学版),2011(5). |
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| 作者姓名： | 齐麟 郜杨 向志明 张彤 |
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| 作者单位： | 中山大学生命科学学院;铁道警官高等专科学校治安系;福州大学生命科学研究所;河南大学生命科学学院; |
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| 基金项目： | 公安部科技创新项目(2010YYCXTDGZ125); 河南省教育厅基础研究项目(200510475016) |
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| 摘 要： | 本文总结了6种DNA抽提方法,进行部分优化后对长时间离体血液痕迹中的DNA进行提取对比.结果表明:优化后的CTAB法可以简单、快速、高效地从凝血块儿中获得高纯度的DNA,并可满足后续PCR扩增和分子标记等分子遗传学研究.
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| 关 键 词： | 血液痕迹 DNA抽提 CTAB PCR ISSR |
Comparison and Optimization of DNA Isolation Methods from Blood Trail |
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| QI Lin,GAO Yang,XIANG Zhi-ming,ZHANG Tong.Comparison and Optimization of DNA Isolation Methods from Blood Trail[J].Journal of Henan University(Natural Science),2011(5). |
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| Authors: | QI Lin GAO Yang XIANG Zhi-ming ZHANG Tong |
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| Institution: | QI Lin1,2,GAO Yang2,XIANG Zhi-ming3,ZHANG Tong4*(1.College of Life Science,Sun yat-sen University,Guangzhou 510275,China,2.Department of Public Security,Railway Police College,Zhengzhou 450003,3.Institute of Life Science,Fuzhou University,Fuzhou 350108,4.College of Life Science,Henan University,Henan Kaifeng 475004,China) |
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| Abstract: | Six methods of DNA isolation from blood trail were performed and analyzed in this paper.Highly purified DNA was simply,fast and efficiently isolated from the blood trail using the optimization CTAB approach.According to PCR and ISSR analysis,the optimization CTAB method was suitable to extract the genomic DNA from blood trail. |
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| Keywords: | blood trail DNA isolation CTAB PCR ISSR |
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