| 福建山樱花的组织培养及植株再生 |
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| 引用本文： | 王光萍,黄敏仁.福建山樱花的组织培养及植株再生[J].南京林业大学学报(自然科学版),2002,26(2):73-75. |
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| 作者姓名： | 王光萍 黄敏仁 |
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| 作者单位： | 南京林业大学林木遗传和基因工程重点实验室,江苏,南京,210037 |
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| 基金项目： | 江苏省高新技术项目 (S0 112 2 9) |
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| 摘 要： | 以春季萌发的福建山樱花嫩枝为外植体诱导丛生芽。在 1 /4MS +BA1 .0mg/L +IBA0 .0 1mg/L的培养基上 ,其丛生芽诱导率达 87.5 % ;在继代培养中选择MS +BA1 .0mg/L +IBA0 .1mg/L +GA30 .3mg/L或KT1 .0mg/L +NAA0 .1mg/L +GA30 .3mg/L培养基 ,不定芽增殖较快。赤霉素对其不定芽的伸长有明显效果 ,当芽伸长至 3～ 4cm时 ,切下置于生根培养基 1 /2MS +NAA1 .0mg/L +IBA1 .0mg/L +BA0 .75mg/L中 ,生根率达 90 %以上 ,移栽成活率达 95 %。
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| 关 键 词： | 福建山樱花 组织培养 植株再生 |
| 文章编号： | 1000-2006(2002)02-0073-04 |
| 修稿时间： | 2001年4月28日 |
Tissue Culture and Plant Regeneration of Cerasus campanulata |
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| WANG Guang ping,HUANG Min ren.Tissue Culture and Plant Regeneration of Cerasus campanulata[J].Journal of Nanjing Forestry University(Natural Sciences ),2002,26(2):73-75. |
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| Authors: | WANG Guang ping HUANG Min ren |
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| Abstract: | Stabilized shoot cultures were achieved when the explant sources were the juvenile branches of Cerasus campanulata in spring.The inducing rate of fasciculated shoots was 87.5% when the inducing medium of 1/4MS with BA 1.0 mg/L and IBA 0.01 mg/L were used.The adventitious shoots were propagated most rapidly when subcultured on the medium of MS with BA 1.0 mg/L,IBA 0.1 mg/L and GA 3 0.3 mg/L,or MS with KT1.0 mg/L,NAA 0.1 mg/L and GA 3 0.3 mg/L.The elongation of adventitious shoots was greatly affected by gibberellin.The adventitious shoots were cut and rooted in the rooting medium of 1/2MS with NAA 1.0 mg/L IBA 1.0 mg/L and BA 0.75 mg/L.The tooting rate was more than 90%.The surviving rate was 95% when the intact shoots were moved to the greenhouse. |
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| Keywords: | Cerasus campanulata Tissue culture Plant regeneration |
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