| 高光效水稻品种对氧化胁迫的响应 |
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| 引用本文： | 朱宏芳,韩宇,孟卫卫,王荣富,靳光远,曹树青. 高光效水稻品种对氧化胁迫的响应[J]. 合肥工业大学学报(自然科学版), 2012, 35(8): 1122-1125 |
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| 作者姓名： | 朱宏芳 韩宇 孟卫卫 王荣富 靳光远 曹树青 |
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| 作者单位： | 1. 合肥工业大学生物与食品工程学院,安徽合肥,230009
2. 安徽农业大学生物技术中心,安徽合肥,230036 |
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| 基金项目： | 安徽高等学校省级自然科学研究重大资助项目(KJ2010ZD04) |
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| 摘 要： | 文章通过对氧化剂甲基紫精处理下高光效水稻杨育粳1号与常规对照品种镇稻11号的鲜重株高的统计、丙二醛含量与叶绿素质量比的测定以及氧化胁迫相关的基因表达分析,研究了水稻的抗氧化机理.研究发现,在20μmol/L甲基紫精处理下,杨育粳1号的鲜重、株高分别是镇稻11号的1.44倍、1.1倍,叶绿素a和b的质量比分别是镇稻11号的2.0倍及2.9倍；活性氧解毒酶mRAN凝胶分析显示,SODA1、APX2及CATB表达量均随甲基紫精浓度增大而增加,且同浓度下前两者在杨育粳1号中的表达量明显高于对照组,据此可推断杨育粳1号抗氧化能力高于镇稻11号.
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| 关 键 词： | 高光效 甲基紫精 氧化胁迫 基因表达 |
Response of rice cultivars with higher photosynthetic efficiency to oxidative stress |
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| ZHU Hong-fang , HAN Yu , MENG Wei-wei , WANG Rong-fu , JIN Guang-yuan , CAO Shu-qing. Response of rice cultivars with higher photosynthetic efficiency to oxidative stress[J]. Journal of Hefei University of Technology(Natural Science), 2012, 35(8): 1122-1125 |
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| Authors: | ZHU Hong-fang HAN Yu MENG Wei-wei WANG Rong-fu JIN Guang-yuan CAO Shu-qing |
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| Affiliation: | 1(1.School of Biotechnology and Food Engineering,Hefei University of Technology,Hefei 230009,China;2.Biological Technology Center,Anhui Agricultural University,Hefei 230036,China) |
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| Abstract: | To illustrate the anti-oxidant mechanism of rice cultivars with higher photosynthetic efficiency to oxidative stress,the effects of methyl viologen treatment to some characters of Yangyujing 1 and Zhendao 11 were investigated,including fresh weight,seedling height,chlorophyll,malondialdehyde and the expression of oxidative stress-related genes.After treated with 20 μmol/L methyl viologen,the fresh weight,seedling height,chlorophyll a and chlorophyll b of Yangyujing 1 were 1.44,1.1,2.0,2.9 times of those of Zhendao 11 respectively.The mRNA gel electrophoresis analysis of active oxygen detoxification enzyme revealed that the expression quantity of SODA1,APX2 and CATB gradiently increased and the increased amount in Yangyujing 1 was significantly more than that in Zhendao 11 especially for SODA1,APX2.Accordingly it could be inferred that the enhanced antioxidant ability of Yangyujing 1 is stronger than that of Zhendao 11 and it may be related to the increased expression of antioxidant genes. |
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| Keywords: | higher photosynthetic efficiency methyl viologen oxidative stress gene expression |
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