| 巴氯芬与胃蛋白酶相互作用的光谱研究 |
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| 引用本文： | 李步海,刘宇飞,孙小梅,王献.巴氯芬与胃蛋白酶相互作用的光谱研究[J].中南民族大学学报(自然科学版),2011,30(1). |
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| 作者姓名： | 李步海 刘宇飞 孙小梅 王献 |
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| 作者单位： | 中南民族大学,化学与材料科学学院分析化学国家民委重点实验室,武汉,430074 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 采用荧光光谱法和紫外可见吸收光谱法研究了巴氯芬与胃蛋白酶的结合.观测到巴氯芬使胃蛋白酶的最大紫外吸收峰蓝移.由Stern-Voliner方程求得其荧光猝灭类型为静态猝灭,求出结合常数,由热力学参数判断其作用力主要为疏水作用.根据非辐射能量转移理论,胃蛋白酶与巴氯芬之间发生了能量转移,从而使胃蛋白酶发生了荧光猝灭.用红外光谱法研究了巴氯芬对胃蛋白酶的影响,结果表明:巴氯芬改变了胃蛋白酶的二级结构.
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| 关 键 词： | 巴氯芬 胃蛋白酶 静态猝灭 光谱法 |
Study of Interaction between Baclofen and Pepsin by Spectroscopy Methods |
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| Li Buhai,Liu Yufei,Sun Xiaomei,Wang Xian.Study of Interaction between Baclofen and Pepsin by Spectroscopy Methods[J].Journal of South-Central Univ for,2011,30(1). |
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| Authors: | Li Buhai Liu Yufei Sun Xiaomei Wang Xian |
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| Institution: | Li Buhai,Liu Yufei,Sun Xiaomei,Wang Xian(Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission,South-central University for Nationalities Wuhan 430074,China) |
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| Abstract: | The binding interaction between baclofen and pepsin was studied by ultraviolet absorption and fluorescence spectroscopies.Baclofen caused maximum of UV absorption peak of pepsin to undergo a blue shift.Stern-Volmer equation indicated that the fluorescence intensity of pepsin was quenched by baclofen via a static quenching mechanism,and the association constants were calculated.The thermodynamic parameters showed that the main binding force was hydrophobic inature.Meanwhile,according to the non-radiative ene... |
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| Keywords: | baclofen pepsin static quenching spectroscopy |
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