Identification of the bioactive peptide PEC-60 in brain |
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Authors: | Å Norberg S Gruber F Angelucci S Renlund H Wadensten S Efendic C-G Östenson H Jörnvall R Sillard A A Mathé |
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Institution: | (1) Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 171 77 Stockholm (Sweden), SE;(2) Department of Physiology and Pharmacology, Karolinska Institutet, 171 77 Stockholm (Sweden), Fax +46 8 331 653, e-mail: aleksander.mathe@knv.ki.se, SE;(3) Amersham Biosciences, 751 25 Uppsala (Sweden), SE;(4) Department of Molecular Medicine, Karolinska Hospital, 171 76 Stockholm (Sweden), SE |
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Abstract: | PEC-60 is a 60-residue peptide originally isolated from pig intestine. It inhibits glucose-induced insulin secretion from
perfused pancreas in a hormonal manner and also has biological activity in the immune system. PEC-60-like immunoreactive material
has been reported in catecholamine neurons of the central and peripheral nervous systems, but the peptide has not been identified
from that material. We have now isolated PEC-60 from pig and rat brains with a method that combines column purification procedures
with the specificity of a radioimmunoassay and the sensitivity of mass spectrometry to directly identify the peptide. The
results show that PEC-60, like many other peptides, is expressed in the gastrointestinal tract and the central nervous system.
The specific regional brain distribution and interaction with classical neurotransmitters raise the possibility that PEC-60may
play a role in the central nervous system disorders involving dopamine dysregulation.
Received 6 December 2002; received after revision 10 December 2002; accepted 11 December 2002
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ID="*"Corresponding author. |
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Keywords: | , PEC-60, radioimmunoassay, brain, pig, rat, |
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