Induction of cell contact sites by Ca2+-EDTA pulses inDictyostelium discoideum |
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| Authors: | S Miyamoto |
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| Institution: | (1) Department of Biology, Tsukuba University, Sakura-mura, Ibaraki, (Japan);(2) Present address: Department of Anatomy, Dokkyo University School of Medicine, Mibu, Tochigi, (Japan) |
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| Abstract: | Summary The effect of extracellular Ca2+ on the differentiation of the cellular slime moldD. discoideum was examined. In the case of sustained application of calcium, cells required at least 60 min for the presence of calcium to show the induction of EDTA resistant cell contact sites (csA). However, the application of 12 pulses of calcium, followed after 30 sec by EDTA, giving a total time of Ca2+-exposure of 6 min, could induce csA on the cell surface. |
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