9～10周龄CB6F1与B6CF1小鼠血生化、主要脏器重量及脏器系数测定

摘要：目的 测定CB6F1与B6CF1小鼠血生化、主要脏器重量和脏器系数，并进行比较分析。方法 随机选取9～10周龄SPF级CB6F1与B6CF1小鼠各40只，雌雄各半。分别称重，麻醉后摘眼球采血后，颈椎脱臼处死，运用全自动生化分析仪测定血生化指标，并采集脏器测定脏器重量，计算脏器系数。 结果 14项血生化指标比较，CB6F1小鼠雌雄之间有11项指标（11/14）、B6CF1小鼠雌雄之间有9项指标（9/14）、CB6F1与B6CF1小鼠雌性之间有10项指标（10/14），雄性之间11项（11/14）指标均存在显著性差异（P﹤0.05）。CB6F1与B6CF1小鼠主要脏器重量与脏器系数比较，同品系雌雄之间，同性别2个品系之间，均有部分指标存在显著性差异（P﹤0.05）。结论 以BALB/c和C57BL/6为不同父母本杂交产生的F1代小鼠CB6F1和B6CF1，其血生化指标、脏器重量和脏器系数有所不同。

Detection of blood biochemical,main organ weight and organ coefficient in 9th to 10th week-old CB6F1 and BCF1 mice

Abstract To detect and compare blood biochemical, main organ weight and organ coefficient of CB6F1 and BCF1 mice. The blood of 40 CB6F1 mice and 40 B6CF1 mice (20 females and 20 males) in the 9th to 10th week were collected by picking eyeball, routine blood indicators were detected by an automatic hematology analyzer, and organ weight to calculate the organ coefficient were determined and analyzed. We compared blood biochemical indices, 11/14 indices were significant difference between male and female of CB6F1 mice, 9/14 indices were significant difference between male and female of B6CF1, 10/14 indices were significant difference between female CB6F1 mice and B6CF1 mice, 11/14 indices were significant difference between male CB6F1 mice and B6CF1 mice male (P < 0.05). Compared main organ weight and organ coefficient of CB6F1 and B6CF1 mice, there were significant difference in some indices for sex and lines (P < 0.05). There were partial differences in the blood biochemical, organ weight and organ coefficient of CB6F1 and BCF1 which were breeding by different female parent and male parent of BALB/c and C57BL/6 mice.