臭氧水对草莓及拟盘多毛孢影响的研究

通过组织分离纯化和分子生物学鉴定，明确了引起草莓叶部新病的致病菌为拟盘多毛孢。以喷洒清水为空白对照，利用不同质量浓度的臭氧水直接喷洒致病菌和生长期的草莓植株，研究臭氧水对致病菌和草莓植株的浓度效应，结果显示：低浓度臭氧水（0.5~0.8 mg·L^-1）对草莓植株的生理生态变化和致病菌的生长影响较小；中浓度臭氧水（2.2~2.5 mg·L^-1）可以显著抑制致病菌的生长，并促进草莓植株的生长；高浓度臭氧水（4.0~4.3 mg·L^-1）可以很好地抑制致病菌的生长，但对草莓叶片有较严重的腐蚀作用。因此，中浓度（2.2~2.5 mg·L^-1）是喷洒草莓的最适臭氧水浓度。

Effect of ozone water on strawberry and Pestalotiopsis

Through the tissue separation, purification and molecular biological identification technologies, it was confirmed that the pathogenic fungus which caused a new disease of strawberry leaves is Pestalotiopsis. Using pure water as a blank control, ozone waters of different concentrations were sprayed to pathogenic fungus directly and to strawberry leaf surface in its growing period.The concentration effects of ozone water on pathogenic fungus and strawberry leaves were studied respectively.The results showed that:lower concentration ozone water(0.5-0.8 mg · L^-1) has little effect on the physiological and ecological changes of strawberry plants and on the growth of pathogenic fungus; medium concentration ozone water(2.2-2.5 mg·L^-1) can significantly inhibit the growth of pathogenic fungus and promote the growth of strawberry; higher concentration ozone water(4.0-4.3 mg·L^-1) can well inhibit the growth of pathogenic fungus.However,it has a serious corrosion effect on strawberry leaves.Therefore,the medium concentration(2.2-2.5 mg·L^-1) is the most suitable ozone water concentration for spraying on strawberry, which can be used in practical production to effectively prevent and control strawberry fungal diseases.