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三七茎叶中γ-氨基丁酸提取工艺研究
引用本文:杨晶晶,曲媛,杨晓艳,杨野,崔秀明.三七茎叶中γ-氨基丁酸提取工艺研究[J].云南大学学报(自然科学版),2014,36(6):907-911.
作者姓名:杨晶晶  曲媛  杨晓艳  杨野  崔秀明
作者单位:1.昆明理工大学 生命科学与技术学院,云南 昆明 650500;
基金项目:云南省教育厅科学研究基金重大专项课题(ZD2014002);昆明理工大学自然科学研究基金(KKSY201326022);昆明理工大学分析测试基金(20140735)
摘    要:探索三七茎叶中γ-氨基丁酸提取工艺.考察提取的溶剂、时间、次数、温度及物料比5个单因素影响,采用正交实验分析方法确定最佳工艺条件.其结果表明三七茎叶中γ-氨基丁酸的最佳提取工艺为:提取溶剂为水,提取时间3 h,提取次数3次,提取温度60 ℃,提取物料比1 g∶10 mL,在此条件下γ-氨基丁酸的提取率为0.46%.

关 键 词:三七茎叶    γ-氨基丁酸(GABA)    提取方法    优化
收稿时间:2014-04-11

Optimization of extraction technique of GABA from the stems and leaves of Panax notoginseng
YANG Jing-jing,QU Yuan,YANG Xiao-yan,YANG Ye,CUI Xiu-ming.Optimization of extraction technique of GABA from the stems and leaves of Panax notoginseng[J].Journal of Yunnan University(Natural Sciences),2014,36(6):907-911.
Authors:YANG Jing-jing  QU Yuan  YANG Xiao-yan  YANG Ye  CUI Xiu-ming
Institution:1.Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,China;
Abstract:In this paper,the optimum conditions for the extraction of GABA from the stems and leaves of Panax notoginseng were determined.On the basis of single-factor experiments(extraction solvent,time,times,temperature,material ratio),the optimum condition for the extraction of GABA was determined by orthogonal experiment.The optimum extraction was based on three times for 3 h at 60 ℃ with water,and the material ratio was 1 g∶10 mL,and the content of GABA from the stems and leaves of Panax notoginseng was 0.46%,and these will provide the technical support for comprehensive development and utilization of the aerial part of Panax notoginseng.
Keywords:stems and leaves of Panax notoginseng    γ-aminobutyric acid(GABA)    extraction technique    optimization  
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