| 藻胆蛋白提取分离及抗氧化活性的测定 |
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| 引用本文： | 陈芳,陈久存.藻胆蛋白提取分离及抗氧化活性的测定[J].陕西理工学院学报(自然科学版),2007,23(2):62-64. |
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| 作者姓名： | 陈芳 陈久存 |
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| 作者单位： | 安康学院,化学与生命科学系,陕西,安康,725000 |
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| 摘 要： | 藻胆蛋白是一种多功能细胞色素蛋白。从条斑紫菜藻中提取藻胆蛋白,用饱和硫酸铵沉淀法、DEAE-纤维素离子交换层析对提取的藻胆蛋白进行纯化,并首次采用邻苯三酚自氧化法来测定藻胆蛋白的抗氧化活性。从而得出:从红藻中提取分离出具有一定纯度、较高抗氧化活力的藻胆蛋白提取品。
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| 关 键 词： | 藻胆蛋 离子交换层析 邻苯三酚自氧化法 抗氧化活性 |
| 文章编号： | 1673-2944(2007)02-0062-03 |
| 修稿时间： | 2006-12-13 |
Purification techniques and detection of the anti-oxidative activity of phycobiliprotein |
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| CHEN Fang,CHEN Jiu-cun.Purification techniques and detection of the anti-oxidative activity of phycobiliprotein[J].Journal of Shananxi University of Technology:Natural Science Edition,2007,23(2):62-64. |
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| Authors: | CHEN Fang CHEN Jiu-cun |
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| Institution: | Chemical and Biological Department, Ankang College, Ankang 725000, China |
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| Abstract: | Phycobiliprotein is a multifunctional cytochome.The study successfully extracted and purified phycobiliprotein from Porphyra yezoensis using saturated solid ammonium sulphated deposition and the DEAE-ion-exchange chromatography.Then pyrogallol autoxidatnion method was used to measure phycobiliproteins' anti-oxidative activity of the extraction phycobiliprotei and comparison of the anti-oxidation of vitamin C with phycobiliproteins at the same concentration was made.The results showed that the phycobiliproteins have highly anti-oxidative activities. |
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| Keywords: | phycobiliprotein ion-exchange chromatography pyrogallol autoxidation method anti-oxidative activity |
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