| 四合木甲醇提取物中杀线虫物质的分离与活性鉴定 |
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| 引用本文： | 刘兵,高雯芳,刘强.四合木甲醇提取物中杀线虫物质的分离与活性鉴定[J].天津师范大学学报(自然科学版),2008,28(1):12-13,32. |
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| 作者姓名： | 刘兵 高雯芳 刘强 |
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| 作者单位： | 天津师范大学,化学与生命科学学院,天津,300387 |
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| 摘 要： | 通过活性追踪，对四合木的甲醇提取物活性成分进行了初步分离，使用快速制备色谱（combiflash companion）对甲醇提取物的氯仿萃取相进行了进一步分离，得到18个较大的馏分，同时以松材线虫（Bursa phelenchus xylophilus）为供试对象，对各馏分杀线虫活性作了初步测定，结果表明：氯仿萃取相F4馏分有较强的杀线虫活性，具有进一步研究的价值．
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| 关 键 词： | 四合木 松材线虫 杀线虫活性 快速制备色谱 |
| 文章编号： | 1671-1114（2008）01-0012-02 |
| 收稿时间： | 2007-01-19 |
| 修稿时间： | 2007年1月19日 |
Separation and bio-active identification of the nematicidal substances from Tetraena mongolica extract |
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| LIU Bing,GAO Wenfang,LIU Qiang.Separation and bio-active identification of the nematicidal substances from Tetraena mongolica extract[J].Journal of Tianjin Normal University(Natural Science Edition),2008,28(1):12-13,32. |
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| Authors: | LIU Bing GAO Wenfang LIU Qiang |
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| Institution: | (College of Chemistry and Life Science, Tianjin Normal University, Tianjin 300387, China) |
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| Abstract: | By using the tracing method the active components of the methanol extract from Tetraena rnongolica were separated, and 18 fractions further separated from the chloroform phase extracting from the methanol extract were obtained by using the combiflash companion, which was in relatively large amounts from different levels in the column. Taking Bursaphelenchus xylophilus as the species, the nematicidal activities of the 18 fractions was preliminarily determined. The results showed that the fraction F4 had an outstanding intensive nematicidal activity against the Bursaphelenchus xylophilus, which would be worthy of further study. |
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| Keywords: | companionTetraena mongolica Bursaphelenchus xylophilus nematicidal activity~ combiflash |
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