沙枣叶片愈伤组织诱导研究初报

以沙枣叶片作为外植体,以MS为基本培养基,用两种不同浓度激素的组合,筛选5组培养基进行继代愈伤组织及增殖诱导.结果表明,沙枣叶片诱导愈伤组织的最佳培养基为MS 6-BA 0.5 mg/L NAA 1 mg/L kT1 mg/L 2,4-D 2 mL 25 g蔗糖 6 g琼脂(pH=5.5),诱导率达86.67%;以MS 6-BA 0.5 mg/L NAA0.5 mg/L kT1 mg/L 2,4-D 2 mL 25 g蔗糖 6 g琼脂(pH=5.5)作为愈伤组织增殖培养较理想.

Report of Induction of Elaeagnus angustifolia L.Callus

We use the blades of Elaeagnus angustifolia L.as the explantation and MS as basic culture medium,select 5 culture mediums to induce new calluses under the combination of two hormones at different concentration.The test result shows that the best culture medium for the induction of callus is MS 6-BA 0.5 mg/L NAA 1mg/L kT 1mg/L 2,4-D 2 mL 25g saccharose 6g agaragar(pH=5.5),the induced rate is 86.67%.Taking MS 6-BA 0.5mg/L NAA 1mg/L kT 1mg/L 2,4-D 2 mL 25g saccharose 6g agaragar(pH=5.5) as callus induction is better mothod.