| 醋渣纤维素降解菌的筛选及鉴定 |
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| 引用本文： | 石春芳,李欣,莫紫琳.醋渣纤维素降解菌的筛选及鉴定[J].科学技术与工程,2018,18(9). |
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| 作者姓名： | 石春芳 李欣 莫紫琳 |
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| 作者单位： | 内蒙古科技大学生命科学与技术学院,内蒙古科技大学生命科学与技术学院,内蒙古科技大学生命科学与技术学院 |
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| 基金项目： | 国家自然科学基金项目（面上项目）；内蒙古科技大学创新基金项目 |
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| 摘 要： | 以包头市呱呱叫调味品厂醋渣为菌源,采用纤维素刚果红培养基、滤纸条崩解试验初筛得到3株降解纤维素活性较高的菌株；采用不同混合菌对醋渣进行固态发酵,考察醋渣的降解率及滤纸酶活性变化,结果表明,H2+H4混合菌对醋渣的降解率最高；采用16SrDNA、18SrDNA序列分析方法对3株菌进行鉴定,菌H2为产黄青霉菌(Penicillium chrysogenum)、菌H4为卷枝毛霉菌(Mucor circinelloides)、菌H16为粪产碱菌(Alcaligenes faecalis)。
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| 关 键 词： | 醋渣 菌种筛选 菌种鉴定 固态发酵 |
| 收稿时间： | 2017/9/5 0:00:00 |
| 修稿时间： | 2017/11/16 0:00:00 |
Screening and Identification of Cellulose Degrading-Bacteria from Vinegar residue |
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| Institution: | Inner Mongolia University of Science & Technology,, |
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| Abstract: | Baotou guaguajiao condiment factory vinegar residue as the bacteria source, using cellulose Congo red medium, the collapse of filter test ,3 strains of Cellulolytic Strains with high activity was screened; using different mixed bacteria for solid-state fermentation of vinegar residue, the degradation rate of vinegar residue and the change of enzyme activity of filter paper were studied, the results showed that the biodegradation of H2+H4 was highest; using 16SrDNA and 18SrDNA sequence analysis to identify 3 strains, strain H2 was Penicillium chrysogenum ,strain H 4 was Mucor circinelloides ,H16 was faecalis Alcaligenes faecalis. |
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| Keywords: | Vinegar residue strain screening identification of bacteria solid-state fermentation |
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