Surface Modification of Anionic Polyurethane with Silk Fibroin Peptide and Its Effects on the Culture of Fibroblasts

The surface modification of the anionicpolyurethane(APU) film was carried out by immersing it insilk fibroin peptide(SFP) solution for 12 h and then treatingwith low temperature plasma glow discharge. The physicalproperties and moisture permeability of modified films wereexamined. The results showed that SFP-modified APU filmshad better moisture permeability than oleophilicpolyurethane, as well as modified APU films kept goodflexibility. Modified APU films could overcome rigid andbrittle weaks of silk fibroin films. The morphology of SFPon the APU film was corpuscular aggregations. The water-contact angle measurement indicated that the change ofhydrophilicity and the element chemical analysis suggestedthat the SFP-modified film surface was enriched withnitrogen atoms. The biocompatibility of APU films may beimproved due to the change of surface compooents. Cellviability and proliferation of rat embryo dermal fibroblastsseeded on control films, APU films and SFP-modified APUfilms were evaluated by MTT assay and viable cell counts,respectively. The results indicated that the APU filmmodified by SFP protein showed the proliferation offibroblasts on the film, and that the compound interface hadgood stability in the air. Results also showed thatpresoaking treatment for APU films was effective toaccomplish the goal of surface modification.