| 科学级冷却型CCD—荧光图像系统定量性能的鉴定 |
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| 引用本文： | 李艳平,李素文.科学级冷却型CCD—荧光图像系统定量性能的鉴定[J].北京师范大学学报(自然科学版),1999,35(3):399-402. |
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| 作者姓名： | 李艳平 李素文 |
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| 作者单位： | 北京师范大学生物学系 |
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| 摘 要： | 以荧光探针Ｈｏｅｃｈｓｔ３３３４２（Ｈｏ３３３４２）特异性标记大白鼠精子和肝细胞ＤＮＡ，用科学冷却型ＣＣＤ显微荧光图像系统获取单个细胞的荧光图像，再以图像处理软件“Ｉｍａｇｅ－Ｐｒｏ ＰＬｕｓ”，分析测量各细胞的ＤＮＡ相对含量和细胞核的相对面积。实验数据表明：鼠肝细胞的ＤＮＡ相对含量分别为精子的ＤＮＡ相对含量的２，４，８倍，符合生物学的规律，说明这套系统的定量结果是可靠的。
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| 关 键 词： | 科学级冷却型 荧光图像系统 电荷耦联装置 CCD |
IDENTIFICATION ABOUT THE QUANTITATIVE FUNCTION OF THE SCIENTIFIC COOLED CCD-FLUORESCENCE IMAGES SYSTEM |
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| Li Yanping, Li Suwen, Cheng Hongyan, Peng Weimin,Zhang Hongqing ,Xue Shaobai.IDENTIFICATION ABOUT THE QUANTITATIVE FUNCTION OF THE SCIENTIFIC COOLED CCD-FLUORESCENCE IMAGES SYSTEM[J].Journal of Beijing Normal University(Natural Science),1999,35(3):399-402. |
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| Authors: | Li Yanping Li Suwen Cheng Hongyan Peng Weimin Zhang Hongqing Xue Shaobai |
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| Abstract: | The DNA of rat spermatzoa and hepatocytes was stained with specifical fluoeescence probe Hoechst33342. The fluoerecence of single cell were archived by the scientific cooled CCD- microscopy fluorescence images spstem DNA relative content and area were analyzed software. The results show that the DNA relative content of rat hepatocytes are 2, 4, 8 times of that of rat spermatozoa respetively, which confoams to the biological law.It indicates that the quantitative result of the system used is reliable. |
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| Keywords: | scientific cooled CCD fluorescence images system rat spermatozoa and hepatocytes DNA relative content ploid |
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