| 蒽酮—硫酸法测定板栗多糖含量 |
| |
| 引用本文： | 李润丰,常学东,狄小丽.蒽酮—硫酸法测定板栗多糖含量[J].河北科技师范学院学报,2010,24(2):54-59. |
| |
| 作者姓名： | 李润丰 常学东 狄小丽 |
| |
| 作者单位： | 河北科技师范学院食品科技学院,河北秦皇岛066600 |
| |
| 摘 要： | 采用蒽酮—硫酸法测定板栗多糖含量并对其测定条件进行优化。结果表明,蒽酮—硫酸法测定板栗多糖的适宜操作条件为:加入2g/L蒽酮—硫酸溶液4mL,在沸水浴中加热3min,自来水冷却40min后在10min内于波长600nm下读取吸光度。蒽酮—硫酸溶液需要现配现用。多糖含量控制在0.02～0.06g/L之间为宜。蒽酮—硫酸法测定板栗多糖含量的精密度实验RSD值为0.792%,平均加样回收率为107.2%,RSD值为4.6%。用本方法测定板栗中多糖含量为12.67%。
|
| 关 键 词： | 板栗多糖 蒽酮—硫酸法 测定 |
Determination of Chestnut Polysaccharide Content with Anthrone-sulfuric Acid Method |
| |
| LI Run-feng,CHANG Xue-dong,DI Xiao-li.Determination of Chestnut Polysaccharide Content with Anthrone-sulfuric Acid Method[J].Journal of Hebei Normal University of Science & Technology,2010,24(2):54-59. |
| |
| Authors: | LI Run-feng CHANG Xue-dong DI Xiao-li |
| |
| Institution: | (College of Food Engineering,Hebei Normal University of Science ＆ Technology,Qinhuangdao Hebei,066600,China) |
| |
| Abstract: | The determination of chestnut polysaccharide content using anthrone-sulfuric acid method was studied and the operating conditions were optimized.The results showed that the optimal operating conditions were：4 mL of 0.2% anthrone-sulfuric acid solution,heated in boiling water bath for 3 min,cooled with city water for 40 min,read the absorbance under 600 nm wavelength in 10 min after cooling.The anthrone-sulfuric acid solution must be used quickly.Polysaccharide concentration between 0.02~0.06 g/L was the best for determination.The RSD in accuracy experiment was 0.792%,and the average recovery rate was 107.2% with RSD of 4.6%.The content of polysaccharide in chestnut determined by this method was 12.67%. |
| |
| Keywords: | Chestnut polysaccharide Anthrone-sulfuric method determination |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
