纳米银与富里酸对AL细胞增殖及产生RNS/ROS的影响

利用ALamar Blue法和RNS/ROS荧光特异性探针,探讨不同浓度纳米银(nAg,0.1~5μg·mL~(-1))、银离子(Ag+,0.01~0.5μg·mL~(-1))及纳米银与富里酸(FA,10μg·mL~(-1))协同作用对人鼠杂交瘤(AL)细胞增殖及活性氮/活性氧自由基产生的影响及在这一过程中的作用.结果显示:在低浓度(小于等于1μg·mL~(-1))情况下,nAg对AL细胞的毒性作用主要来自于nAg释放的银离子(Ag~+),Ag~+通过诱导胞内RNS的产生,进而产生增殖抑制作用;在高浓度(5μg·mL~(-1))情况下,nAg对AL细胞的毒性主要来自于Ag~+诱导的RNS和nAg本身诱导的ROS二者共同的作用.模拟水环境中存在的FA(10μg·mL~(-1))能够显著降低nAg和Ag~+对AL细胞增殖的抑制作用,并且能显著降低nAg和Ag~+引起的A_L细胞内RNS和ROS的升高作用.

Effect of silver nanoparticles and FA on the proliferation and RNS/ROS production of AL cells

Using ALamar Blue assay and ROS/RNS fluorescent probe, we studied the cytotoxicity of silver nanoparticles (0.1-5 μg·mL-1 nAg), silver ion (0.01-0.5 μg·mL-1 Ag+) and nAg associated with fulvic acid (10 μg·mL-1 FA) of Human-hamster hybrid AL cells in vitro.The results showed that silver ions released from nAg solution had much more effect on cell viability than nAg at low concentrations, and the inhibition was mainly through the induction of RNS by silver ions.However, at high concentrations nAg had much more effect on cell viability, and this inhibition was mainly due to the induction of RNS by silver ions and ROS by silver nanoparticles.The results also showed that FA could significantly recover the cellular viability decreased by silver nanoparticles and silver ion, as well as the induction of RNS/ROS.