| 超高效液相色谱-串联质谱法检测禽畜肉中的氯霉素残留 |
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| 引用本文： | 尹丽珠,张学忠,冯雷,珠娜,祝红昆,牛之瑞.超高效液相色谱-串联质谱法检测禽畜肉中的氯霉素残留[J].云南大学学报(自然科学版),2011,33(3):336-339. |
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| 作者姓名： | 尹丽珠 张学忠 冯雷 珠娜 祝红昆 牛之瑞 |
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| 作者单位： | 云南省产品质量监督检验研究院; |
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| 基金项目： | 云南省科技平台项目资助(2009DA022) |
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| 摘 要： | 研究了一种针对禽畜肉中氯霉素残留量的超高效液相色谱-串联质谱联用分析方法.本方法采用乙酸乙酯萃取禽畜肉中的氯霉素,提取液氮吹至干后用超纯水溶解样品残渣,然后用HLB小柱进行固相萃取,经过净化的样品通过超高效液相色谱进行分离,然后用电喷雾质谱负离子多反应监测模式对氯霉素进行检测.该方法的检出限为0.0025μg/kg,添加水平为0.1~5.0μg/kg时,加标回收率为75.8%~82.5%,相对标准偏差小于10%.该方法具有灵敏度高、选择性好、分析时间短等优点.
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| 关 键 词： | 氯霉素 禽畜肉 超高效液相色谱-串联质谱联用 分析 |
| 收稿时间： | 2010-5-28 |
Determination of chloramphenicol residues in meat by UPLC-MS/MS |
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| YIN Li-zhu,ZHANG Xue-zhong,FENG Lei,ZHU Hong-kun,ZHU Na,NIU Zhi-rui.Determination of chloramphenicol residues in meat by UPLC-MS/MS[J].Journal of Yunnan University(Natural Sciences),2011,33(3):336-339. |
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| Authors: | YIN Li-zhu ZHANG Xue-zhong FENG Lei ZHU Hong-kun ZHU Na NIU Zhi-rui |
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| Institution: | YIN Li-zhu,ZHANG Xue-zhong,FENG Lei,ZHU Hong-kun,ZHU Na,NIU Zhi-rui(Yunnan Institute of Product Quality Supervision and Inspection,Kumming 650223,China) |
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| Abstract: | A new determination of chloramphenicol in meat was introduced.Solid-phase extraction(SPE) was applied in pretreatment of samples which were separated by ultra performance liquid chromatography(UPLC) with acetronitrile-water as mobile phase afterwards.The detection of chloramphenicol residues was performed by MS/MS in ESI(-)-MRM mode.The results shows that the pretreatment,as well as the analytical approach,was highly efficient,sensitive and also specific to chloramphenicol. |
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| Keywords: | chloramphenicol UPLC-MS/MS meat |
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