| 人肿瘤坏死因子穿梭表达载体的构建 |
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| 引用本文： | 王捷.人肿瘤坏死因子穿梭表达载体的构建[J].华南理工大学学报(自然科学版),1998,26(4):82-85. |
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| 作者姓名： | 王捷 |
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| 作者单位： | 华南理工大学生物工程研究所，广州军区医学实验中心，中科院植物研究所 |
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| 摘 要： | 应用ＤＮＡ重组技术，将人肿瘤坏死因子（ｒｈＴＮＦ）ｃＤＮＡ插到质粒ＰＲＬ４３９的ＰｐｓｂＡ启动子下游，得到中间载体ＰＲＬ＿ＴＮＦ；再把ＰＲＬ＿ＴＮＦ上含ＰｐｓｂＡ和（ｒｈＴＮＦ）ｃＤＮＡ的片段连到穿梭载体ＰＤＣ＿８上，构建成穿梭表达载体ＰＤＣ＿ＴＮＦ．转化大肠杆菌ＴＧ１后，进行发酵培养．ＳＤＳ＿ＰＡＧＥ分析和免疫印迹检测结果显示ｒｈＴＮＦ获稳定表达，分子量为１７ｋｕ．本研究结果为ｒｈＴＮＦ在蓝藻中的表达提供了技术基础．
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| 关 键 词： | 人肿瘤坏死因子 穿梭载体 重组 |
CONSTRUCTION OF SHUTTLE VECTOR EXPRESSING THE RECOMBINANT HUMAN TUMOR NECROSIS FACTOR |
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| Wang Jie Jiang Yuehua Guo Yong Shi Dingji,Zhang Hongbin,Liu Fenglong.CONSTRUCTION OF SHUTTLE VECTOR EXPRESSING THE RECOMBINANT HUMAN TUMOR NECROSIS FACTOR[J].Journal of South China University of Technology(Natural Science Edition),1998,26(4):82-85. |
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| Authors: | Wang Jie Jiang Yuehua Guo Yong Shi Dingji Zhang Hongbin Liu Fenglong |
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| Institution: | Wang Jie Jiang Yuehua * Guo Yong Shi Dingji ** Zhang Hongbin * Liu Fenglong ** |
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| Abstract: | Human tumor necrosis factor (rhTNF) cDNA was inserted downstream of PpsbA promoter on the plasmid PRL439 to get the intermediate plasmid PRL_TNF. Then, the fragment containing PpsbA and rhTNF cDNA was linked with the shuttle vector PDC_8, and resulted in expressing vector PDC_TNF. PDC_TNF was then transformed into a E. coli strain TG 1. After the culture, we found the expression of the rhTNF in E. coli TG 1 with apparent molecular weight of 17 ku. The results will help the study of the expression of rhTNF in the cyanobacteria. |
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| Keywords: | rhTNF shuttle vector recombinant |
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