|
|||||
|
|
| 新型HLA-A2限制的B, C型HBV特异性CTL表位的筛选及鉴定 | |
| 引用本文： | 任玉林,张宇,邱立鹏,张小俊,陈煜,王福生,谢尧,高斌,段钟平,孟颂东.新型HLA-A2限制的B, C型HBV特异性CTL表位的筛选及鉴定[J].科学通报,2010,55(30):2915-2924. |
| 作者姓名： | 任玉林 张宇 邱立鹏 张小俊 陈煜 王福生 谢尧 高斌 段钟平 孟颂东 |
| 作者单位： | 中国科学院微生物研究所, 北京 100101; 中国科学技术大学生命科学学院, 合肥 230026; 首都医科大学附属佑安医院, 北京 100069; 中国人民解放军第302医院, 北京 100039; 北京市地坛医院, 北京100015 |
| 基金项目： | 国家自然科学基金(30970146)和国家“十一五”传染病重大专项课题(2008ZX10002-005-3)资助项目 |
| 摘 要： | 乙型肝炎病毒(HBV)特异性CD8+ T细胞(CTL)介导的细胞免疫应答在控制HBV感染和病毒清除中至关重要, 已鉴定的T细胞表位绝大多数都来自A, D基因型的乙肝病毒, 而在我国流行的B, C型病毒表位研究较少. 本研究合成重叠九肽肽库, 通过细胞结合试验和体外重折叠实验系统筛选和鉴定B型和C型HBV核心蛋白表位, 发现一个在60位氨基酸由L变异成V(L60V)而产生的新表位HBcAg60-68. 表位肽能在HLA-A2/Kb转基因小鼠体内激发特异性CTL细胞免疫反应. 检测HLA-A2阳性乙肝患者表位特异性CTL证明该九肽序列为体内自然加工的表位, 这为研究HBV感染中特异性CTL免疫应答提供新的依据. |
| 关 键 词： | HBV 表位 突变 HLA-A2/Kb 转基因 |
| 收稿时间： | 2010-03-31 |
Identification of an HLA-A2 restricted HBcAg-specific T cell epitope derived from HBV genotypes B and C |
|
| REN YuLin,ZHANG Yu,QIU LiPeng,ZHANG XiaoJun,CHEN Yu,WANG FuSheng,XIE Yao,GAO Bin,DUAN ZhongPing & MENG SongDong.Identification of an HLA-A2 restricted HBcAg-specific T cell epitope derived from HBV genotypes B and C[J].Chinese Science Bulletin,2010,55(30):2915-2924. | |
| Authors: | REN YuLin ZHANG Yu QIU LiPeng ZHANG XiaoJun CHEN Yu WANG FuSheng XIE Yao GAO Bin DUAN ZhongPing & MENG SongDong |
| Institution: | 1 Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; 2 School of Life Science, University of Science and Technology of China, Hefei 230026, China; 3 Beijing You’an Hospital, Capital Medical University, Beijing 100069, China; 4 302 Military Hospital of China, Beijing 100039, China; 5 Beijing Ditan Hospital, Beijing 100015, China |
| Abstract: | Hepatitis B virus (HBV)-specific CD8+ T lymphocytes (CTL) play a major role in the control and clearance of HBV infection. However, the majority of human leukocyte antigen (HLA) class I restricted epitopes have been defined in HBV genotypes A and D, but definitions are lacking in genotypes B and C, which are prevalent in China. In this study, the overlapping 9-mer peptide pools were used to screen and identify genotypes B- and C-derived HBcAg-specific T cell epitopes by HLA stabilization assays and in vitro re-folding assays. We identified a new epitope derived from a leucine to valine mutation at position 60 of the core protein (L60V). This epitope can elicit CTL responses in HLA-A2/Kb transgenic mice. The defined HBV epitope was further characterized by investigating specific CTL responses in HLA-A2 positive patients with acute and chronic hepatitis B, indicating that the T cell epitope was naturally processed. These findings will provide a basis for the analysis of CTL responses in patients with HBV infection. |
| Keywords: | hepatitis B virus epitope mutation HLA-A2 CTL HLA-A2/Kb transgenic mice |
| 点击此处可从《科学通报》浏览原始摘要信息 | |
| 点击此处可从《科学通报》下载免费的PDF全文 | |