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酵母胞内海藻糖微波破细胞提取与传统提取比较
引用本文:刘传斌,李宁,白凤武,苗蔚荣,苏志国.酵母胞内海藻糖微波破细胞提取与传统提取比较[J].大连理工大学学报,2001,41(2):169-172.
作者姓名:刘传斌  李宁  白凤武  苗蔚荣  苏志国
作者单位:1. 大连理工大学生化工程研究所, 辽宁大连 116012
2. 大连理工大学染料、表面活性剂精细化工合成国家重点实验室, 辽宁大连 116012
3. 中国科学院化工冶金研究所生化工程国家重点实验室, 北京 100080
摘    要:酵母胞内海藻糖大都采用 50 %乙醇 -水溶液回流提取得到 .但酵母经微波破细胞处理后 ,以水为溶剂在室温下提取 1 0 min,就能把海藻糖充分溶出 .与传统浸取相比较 ,微波破细胞提取具有提取时间短、不需有机溶剂、不需加热、海藻糖收率高、杂质溶出少等优点

关 键 词:海藻糖  提取/微波破碎细胞
文章编号:1000-8608(2001)02-0169-04
修稿时间:2000年4月16日

Comparison of trehalose extraction from yeast after microwave cell disruption and traditional method
LIU Chuan bin,LI Ning,BAI Feng wu,MIAO Wei rong,SU Zhi guo.Comparison of trehalose extraction from yeast after microwave cell disruption and traditional method[J].Journal of Dalian University of Technology,2001,41(2):169-172.
Authors:LIU Chuan bin  LI Ning  BAI Feng wu  MIAO Wei rong  SU Zhi guo
Institution:LIU Chuan bin1,LI Ning1,BAI Feng wu1,MIAO Wei rong2,SU Zhi guo3
Abstract:Extraction of trehalose from yeast was mainly carried out by 50% ethanol water solution at boiling point. However, to the yeast after microwave cell disruption, trehalose could be easily extracted by water at room temperature in 10 min.. Compared with the traditional leaching operation, the usage of the organic solvent and long time heating were avoided, less impurity was extracted, and the extraction time was shortened in the extraction of trehalose after microwave cell disruption.
Keywords:trehalose  extraction/microwave cell disruption
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