苦荞籽中黄曲霉毒素B1质量分数检测方法的研究

利用AFB1免疫亲和柱对苦荞籽进行前处理,使用高效液相色谱仪测定AFBl的质量浓度,建立了一种检测苦荞籽中AFB1质量分数的检测方法。采用该方法测定了凉山州不同区域苦荞籽中AFB1的质量分数。结果表明:新建方法的检测限为0.2 ng/ ml,精密度良好,添加回收率平均为85.87%。利用此法测定了凉山州不同区域的苦荞籽,在凉山州不同地区收集的苦荞籽中均未检出AFB1。

Study on the Monitoring Method of Aflatoxin B1 Content in Tartary Buckwheat Weeds

In order to establish a feasible method for monitoring aflatoxin B1 in ''Tartary buckwheat seedls , aflatoxin B1 im-munoaffinity column combined with high performance liquid chromatography is used to determine the content of aflatoxinBl in ''Tartary buckwheat seeds.The standard curve was drawn.The detection limit, and added recovery of the method weredetermined.The results show that the detection limits of the new method is 0.20 ng/mL, the precision is good ,and the aver-age recovery nate was 85.87%.The aflatoxin Bl contents of Tartary buckwheat seeds in different areas of Liangshan Prefec-ture are detected.The results show that the seeds of Tartary buckwheat collected from different areas of Liangshan Prefec-ture do not contain aflatoxin B1.