| 用PCR法检测人巨细胞病毒DNA |
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| 引用本文： | 袁仕取,刘明霞.用PCR法检测人巨细胞病毒DNA[J].陕西师范大学学报,1996,24(2):77-79. |
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| 作者姓名： | 袁仕取 刘明霞 |
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| 作者单位： | 陕西师范大学生物学系，华美生物工程公司 |
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| 摘 要： | 用聚合酶链式反应（ＰＣＲ）法检测患者尿液中人巨细胞病毒（ＨＣＭＶ）ＤＮＡ．结果表明，自行设计合成的引物位于ＨＣＭＶ基因组早期蛋白基因区，经ＰＣＲ仪扩增一段长４３０ｂｐ的特异序列片段，对正常人基因组ＤＮＡ或其它疱疹病毒ＤＮＡ无交叉反应．此法可检测出少至１０－１６ｇ（０．１ｆｇ）的病毒ＤＮＡ．通过对３５份尿液标本的检测，比较ＰＣＲ法和组织培养法的检测结果完全一致
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| 关 键 词： | 人巨细胞病毒 聚合酶链式反应 临床医学 |
Detection of human cytomegalovirus DNA by polymerase chain reaction |
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| Yuan Shiqu ,Liu Mingxia ,Li Zhenyong ,Liu Junjian.Detection of human cytomegalovirus DNA by polymerase chain reaction[J].Journal of Shaanxi Normal University: Nat Sci Ed,1996,24(2):77-79. |
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| Authors: | Yuan Shiqu Liu Mingxia Li Zhenyong Liu Junjian |
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| Institution: | Yuan Shiqu 1,Liu Mingxia 2,Li Zhenyong 2,Liu Junjian 1 |
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| Abstract: | PCR method is used to detect HCMV DNA in patients urine. A pair of primers are designed in the site of early protein gene of HCMV genome. It could be used to amplify a 430 bp length sequence of HCMV which has no cross reaction with human genomic DNA and Herpesvirus DNA. HCMV DNA which weigh as light as 10 -16 g(0.1fg) could be detected. By assaying 35 urine specimens. The result agress with that of Tissue Culture and demonstrats that the PCR assay is very useful to clinical apply. |
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| Keywords: | HCMV PCR clinical medicine |
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