| 免疫亲和层析纯化兔抗人IgG |
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| 引用本文： | 王继华,李文美,吴革.免疫亲和层析纯化兔抗人IgG[J].华南理工大学学报(自然科学版),1997(11). |
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| 作者姓名： | 王继华 李文美 吴革 |
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| 作者单位： | [1]暨南大学医学院生化教研室 [2]华南理工大学万孚生物技术公司 [3]广州市医药卫生研究所 广州 |
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| 摘 要： | 用人IgG-Sepharose 4B亲和柱,3mol/L氯化镁洗脱,一步纯化免抗人IgG血清,获得了SDS-PAGE电泳纯,免疫双扩散法效价达1/256,收率62.6%;用8mol/L脲洗脱,效价为1/128,收率34.1%.与硫酸铵多步沉淀法比较,纯度和效价大大提高,为获得高度特异、高亲和力兔抗人IgG提供了一条简便途径.
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| 关 键 词： | 兔抗人IgG 亲和层析 氯化镁 |
PURIFICATION OF RABBIT ANTI-HUMAN IgG WITH AFFINITYCHROMATOGRAPHY |
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| Wang Jihua Li Wenmei Wu Ge.PURIFICATION OF RABBIT ANTI-HUMAN IgG WITH AFFINITYCHROMATOGRAPHY[J].Journal of South China University of Technology(Natural Science Edition),1997(11). |
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| Authors: | Wang Jihua Li Wenmei Wu Ge |
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| Abstract: | Using humanlgG-sepharose 4B affinitychromatography column with 3 mol/L MgCl2 eluant, the rabbit anti-human IgG antisera were isolated in one step. The results revealed that the rabbit anti-human IgG was pure in SDS-PAGE, its titer was 1:256 in IDD, the yield was 62. 6% . Replacing 3 mol/L MgCl2 with 8 mol/L urea, the titer in IDD was 1:128, the yield was 34.1%. Camparing with (NH4)2SO4 precipitations, using the affinitychromatography, we can obtained a higher specificity and affinity rabbit anti-human IgG, it is a simple and effective method. |
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| Keywords: | rabbit anti-human IgG affinitychromatography magnesium chloride |
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