基于Plackett—Burman设计的刺糖多孢菌培养条件优化

多杀菌素是新型生物源农药，系刺糖多孢菌的次级代谢产物。在单因子实验基础上，采用Plackett—Burman实验设计方法，优化了刺糖多孢菌培养条件。结果筛选出初始pH值、培养温度、摇瓶转速、摇瓶装液量4个因子，为本实验奈件下影响刺糖多孢菌生物量的主效因子，4因子贡献率总和达90．8％，建立了回归模型Y=7．73-0．63x1＋0．42x5-0．20x7＋0．20x8，决定系数R^2=0．9767，调整决定系数R^2=0．9359，方差分析表明模型显著，生物量最大预测值可达9．18g／L。

Optimization of Culture Conditions of Saccharopolyspora Spinosa Based on Plackett-Burman Design

Spinosad is a novel biological pesticide, which is secondary metabolites of Saccharopolyspora spinosa. On the basis of single factor experiments, optimization of culture conditions of Saccharopolyspora spinosa was studied by Plackett-Burman design.- Four main effect factors were screened out, including initial pH value, culture temperature, shaking speed, liquid volume, and their contribution to the model amounts to 90. 8%. Tile model was established, Y= 7. 73-0. 63x1 ＋0. 42x5 -0. 20x7 ＋0. 20x8, which was significant model by analysis of variance. The model＇s determination coefficient R-Squared was 0. 9767 and adjusted determination coefficient R-Squared was 0. 9359. According to the model, maximum predictive value of Saccharopolyspora spinosa biomass is 9. 18 g/L.